TY - JOUR
T1 - Correlating Fluorescence and High-Resolution Scanning Electron Microscopy (HRSEM) for the study of GABAA receptor clustering induced by inhibitory synaptic plasticity
AU - Orlando, Marta
AU - Ravasenga, Tiziana
AU - Petrini, Enrica Maria
AU - Falqui, Andrea
AU - Marotta, Roberto
AU - Barberis, Andrea
N1 - KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: We thank R. Ruffilli for her support in performing CL-HRSEM and ET experiments. RM is also grateful to A. Genovese and A. Casu for their friendship. This work has been supported by Telethon-Italy (GGP16083 to A.B.) and Compagnia di San Paolo (Grant ROL-9687 to Marti Duocastella and A.B.).
PY - 2017/10/23
Y1 - 2017/10/23
N2 - Both excitatory and inhibitory synaptic contacts display activity dependent dynamic changes in their efficacy that are globally termed synaptic plasticity. Although the molecular mechanisms underlying glutamatergic synaptic plasticity have been extensively investigated and described, those responsible for inhibitory synaptic plasticity are only beginning to be unveiled. In this framework, the ultrastructural changes of the inhibitory synapses during plasticity have been poorly investigated. Here we combined confocal fluorescence microscopy (CFM) with high resolution scanning electron microscopy (HRSEM) to characterize the fine structural rearrangements of post-synaptic GABAA Receptors (GABAARs) at the nanometric scale during the induction of inhibitory long-term potentiation (iLTP). Additional electron tomography (ET) experiments on immunolabelled hippocampal neurons allowed the visualization of synaptic contacts and confirmed the reorganization of post-synaptic GABAAR clusters in response to chemical iLTP inducing protocol. Altogether, these approaches revealed that, following the induction of inhibitory synaptic potentiation, GABAAR clusters increase in size and number at the post-synaptic membrane with no other major structural changes of the pre- and post-synaptic elements.
AB - Both excitatory and inhibitory synaptic contacts display activity dependent dynamic changes in their efficacy that are globally termed synaptic plasticity. Although the molecular mechanisms underlying glutamatergic synaptic plasticity have been extensively investigated and described, those responsible for inhibitory synaptic plasticity are only beginning to be unveiled. In this framework, the ultrastructural changes of the inhibitory synapses during plasticity have been poorly investigated. Here we combined confocal fluorescence microscopy (CFM) with high resolution scanning electron microscopy (HRSEM) to characterize the fine structural rearrangements of post-synaptic GABAA Receptors (GABAARs) at the nanometric scale during the induction of inhibitory long-term potentiation (iLTP). Additional electron tomography (ET) experiments on immunolabelled hippocampal neurons allowed the visualization of synaptic contacts and confirmed the reorganization of post-synaptic GABAAR clusters in response to chemical iLTP inducing protocol. Altogether, these approaches revealed that, following the induction of inhibitory synaptic potentiation, GABAAR clusters increase in size and number at the post-synaptic membrane with no other major structural changes of the pre- and post-synaptic elements.
UR - http://hdl.handle.net/10754/625933
UR - https://www.nature.com/articles/s41598-017-14210-5
UR - http://www.scopus.com/inward/record.url?scp=85032195002&partnerID=8YFLogxK
U2 - 10.1038/s41598-017-14210-5
DO - 10.1038/s41598-017-14210-5
M3 - Article
C2 - 29061992
SN - 2045-2322
VL - 7
JO - Scientific Reports
JF - Scientific Reports
IS - 1
ER -