TY - JOUR
T1 - Correlative Raman–Electron–Light (CREL) Microscopy Analysis of Lipid Droplets in Melanoma Cancer Stem Cells
AU - Pagliari, Francesca
AU - Sogne, Elisa
AU - Panella, Davide
AU - Perozziello, Gerardo
AU - Liberale, Carlo
AU - Das, Gobind
AU - Turdo, Alice
AU - Di Franco, Simone
AU - Seco, Joao
AU - Falqui, Andrea
AU - Gratteri, Santo
AU - Pujia, Arturo
AU - Di Fabrizio, Enzo
AU - Candeloro, Patrizio
AU - Tirinato, Luca
N1 - KAUST Repository Item: Exported on 2022-12-06
Acknowledgements: L.T. received funding from AIRC and the European Union’s Horizon 2020 Research and Innovation Programme under the Marie Skłodowska-Curie grant agreement N° 800924. We are grateful to Federico Mecarini for his precious support and advice during SEM measurement.
PY - 2022/12/1
Y1 - 2022/12/1
N2 - Among all neoplasms, melanoma is characterized by a very high percentage of cancer stem cells (CSCs). Several markers have been proposed for their identification, and lipid droplets (LDs) are among them. Different techniques are used for their characterization such as mass spectrometry, imaging techniques, and vibrational spectroscopies. Some emerging experimental approaches for the study of LDs are represented by correlative light–electron microscopy and by correlative Raman imaging–scanning electron microscopy (SEM). Based on these scientific approaches, we developed a novel methodology (CREL) by combining Raman micro-spectroscopy, confocal fluorescence microscopy, and SEM coupled with an energy-dispersive X-ray spectroscopy module. This procedure correlated cellular morphology, chemical properties, and spatial distribution from the same region of interest, and in this work, we presented the application of CREL for the analysis of LDs within patient-derived melanoma CSCs (MCSCs).
AB - Among all neoplasms, melanoma is characterized by a very high percentage of cancer stem cells (CSCs). Several markers have been proposed for their identification, and lipid droplets (LDs) are among them. Different techniques are used for their characterization such as mass spectrometry, imaging techniques, and vibrational spectroscopies. Some emerging experimental approaches for the study of LDs are represented by correlative light–electron microscopy and by correlative Raman imaging–scanning electron microscopy (SEM). Based on these scientific approaches, we developed a novel methodology (CREL) by combining Raman micro-spectroscopy, confocal fluorescence microscopy, and SEM coupled with an energy-dispersive X-ray spectroscopy module. This procedure correlated cellular morphology, chemical properties, and spatial distribution from the same region of interest, and in this work, we presented the application of CREL for the analysis of LDs within patient-derived melanoma CSCs (MCSCs).
UR - http://hdl.handle.net/10754/686176
UR - https://www.mdpi.com/2079-6374/12/12/1102
U2 - 10.3390/bios12121102
DO - 10.3390/bios12121102
M3 - Article
C2 - 36551069
SN - 2079-6374
VL - 12
SP - 1102
JO - Biosensors
JF - Biosensors
IS - 12
ER -
