Corrigendum to: Structural basis for specific inhibition of the highly sensitive Sh HTL 7 receptor (EMBO reports, (2018), 19, 9, 10.15252/embr.201745619)

Correction to: EMBO Reports (2018) 19: e45619. DOI: 10.15252/embr.201745619 | Published online 18 July 2018 The authors have requested to retract and replace Figs 4C and 7C, and to update the sections in the text based on the corrected data. (Figure presented.) Figure 4C. Original. Figure 4C: Triton X-100 and n-dodecyl-β-D-maltoside were applied at two different concentrations in the presence of four different concentrations of GR24. In the published paper, the data are shown as box-and-whisker plots with minimum, first quartile (Q1), median, third quartile (Q3), and maximum. The median value (Q2/50th percentile) corresponds to the middle value of the pooled dataset obtained for the four GR24 concentrations, which is misleading since the plot does not display the dose–response of Striga germination to the four different concentrations of GR24 and the relative inhibition by the two compounds. The data are now presented separately for each GR24 concentration. The updated Fig 4C shows Striga seed germination ratios in response to two concentrations of Triton X-100 (1.54 μM and 15.4 μM) and n-dodecyl-β-D-maltoside (1.5 μM and 15.0 μM) along with 4 different concentrations of GR24 (1, 0.5, 0.25, and 0.125 nM). The conclusion that the application of Triton X-100 at a concentration of 15.4 μM results in an approx. 50% reduction in GR24-induced germination remains unchanged. In addition, the authors state they erroneously used control values from a parallel experiment in the published Fig 4C (the data obtained with n-dodecyl-β-D-maltoside were from a different set of experiments than the displayed control and Triton X-100 data). The new Fig 4C depicts the data obtained from the same set of experiments and thus the correct values of the effect of n-dodecyl-β-D-maltoside on Striga seed germination. The authors had previously stated that the application of the detergent n-dodecyl-β-D-maltoside did not affect Striga seed germination. Based on the correct control now depicted in new Fig 4C, this statement needs to be corrected from “This result is not explained by a general inhibitory effect of detergent on Striga seeds, because under the same conditions, the application of the detergent n-dodecyl-β-D-maltoside did not affect germination (Fig 4C)”. to “n-dodecyl-β-D-maltoside showed about 10-20% inhibition of Striga seed germination (Fig 4C)”. (Figure presented.) Figure 4C. Corrected. The legend of Fig 4C has been updated from “Percentage of Striga hermonthica seeds germinating after 2 days of treatment with Triton at 15.4 (0.001%) and 1.54 µM (0.0001%) concentrations and n-dodecyl-β-D-maltoside at 15 and 1.5 µM concentrations in the presence of GR24 at 1, 0.5, 0.25, and 0.125 nM concentrations, respectively. Data are shown as box and whisker plots, where horizontal central value represents median, upper and lower quartiles are the ends of the box, the box spans the interquartile range and whiskers are the maximum and minimum values, n = 6 (50–100 seeds per replicate), *P < 0.05, **P < 0.01 (one-way ANOVA)”. to “Percentage of Striga hermonthica seeds germinating after 2 days of treatment with Triton X-100 at 15.4 and 1.54 µM concentrations and n-dodecyl-β-D-maltoside at 15.0 and 1.5 µM concentrations in the presence of GR24 at 1.0, 0.5, 0.25, and 0.125 nM concentrations, respectively. The data/error bars represent the mean/standard error of six replicate measurements (50–100 seeds per replicate)”. (Figure presented.) Figure 7. Original. Figure 7C: Phenoxyacetic acid (PAA) was applied at two different concentrations in the presence of four different concentrations of GR24. Similar to Figure 4C, the results as presented were misleading box-and-whisker plots that did not indicate the different concentrations of GR24. In the corrected figure, the data are now plotted for each GR24 concentration separately and show Striga seed germination ratios in response to the applied concentrations (1.0 μM and 10.0 μM) of 2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]acetic acid along with 4 different concentrations of GR24 (1.0, 0.5, 0.25, and 0.125 nM). The legend of Fig 7C has been updated from (Figure presented.) Figure 7C. Corrected. “Percentage of Striga hermonthica seeds germinating after 2 days of treatment with 2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]acetic acid at 10 and 1 µM in the presence of GR24 at 1, 0.5, 0.25, and 0.125 nM concentrations, respectively. Data are shown as box and whisker plots, where horizontal central value represents median, upper and lower quartiles are the ends of the box, the box spans the interquartile range and whiskers are the maximum and minimum values, n = 6 (50–100 seeds per replicate), *P < 0.05 (one-way ANOVA)”. to “Percentage of Striga hermonthica seeds germinating after 2 days of treatment with 2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]acetic acid at 10.0 and 1.0 µM concentrations in the presence of GR24 at 1.0, 0.5, 0.25, and 0.125 nM concentrations, respectively. The data/error bars represent the mean/standard error of six replicate measurements (50–100 seeds per replicate)”. The source data for the Striga germination assays have been deposited on Biostudies with the accession number S-BIAD173 [https://www.ebi.ac.uk/biostudies/studies/S-BIAD173]. In addition to these changes, the authors are correcting the following: In Materials and Methods, the amount of reduced glutathione in the section “Cloning, expression and protein purification” is corrected from 0.2 mM to 20 mM. The concentration of purified ShHTL7, ShHTL7-L143Y, AtD14, and OsD14 protein used in the section “Yoshimulactone green (YLG) hydrolysis assays;” is corrected from 3 μM to 0.3 μM. The preincubation time of protein and Triton X-100 in the section “Yoshimulactone green (YLG) hydrolysis assays; Materials and Methods” is corrected from 30 min to 2 h 30 min. The conclusion that Triton X-100 binds ShHTL7 with an IC₅₀ of 0.47 ± 0.11 μM remains unchanged. The authors USH, IH, MJ, XG, RAZ, DK, SAB, and STA agree with this correction. BAK does not agree with this correction due to concerns he has about the data and recuses himself from authorship. The updated authorship of the article is: Umar Shahul Hameed, Imran Haider, Muhammad Jamil, Xianrong Guo, Randa A Zarban, Dongjin Kim, Salim Al-Babili, and Stefan T Arold. The authors apologize for not detecting these errors prior to publication and for any inconvenience that they may have caused.