TY - JOUR
T1 - Cost effective surface functionalization of silver nanoparticles for high yield immobilization of Aspergillus oryzae β-galactosidase and its application in lactose hydrolysis
AU - Ansari, Shakeel Ahmed
AU - Satar, Rukhsana
AU - Alam, Fahad
AU - Alqahtani, Mohammed Husein
AU - Chaudhary, Adeel Gulzar
AU - Naseer, Muhammad Imran
AU - Karim, Sajjad
AU - Sheikh, Ishfaq Ahmed
N1 - Generated from Scopus record by KAUST IRTS on 2023-09-23
PY - 2012/12/1
Y1 - 2012/12/1
N2 - The present study demonstrates synthesis, characterization and surface functionalization of silver nanoparticles (AgNPs) via glutaraldehyde for high yield immobilization of Aspergillus oryzae β-galactosidase. Soluble β-galactosidase (SβG), enzyme adsorbed on unmodified AgNPs (UβG) and surface modified AgNPs (MβG) showed same pH-optima at pH 4.5. However, it was observed that MβG exhibited enhanced pH stability toward acidic and alkaline sides, and increased temperature resistance as compared to SβG and UβG. Michaelis constant, Km was increased nearly three-folds for MβG while Vmax for soluble and MβG was 0.515 mM/min and 0.495 mM/min, respectively. Furthermore, MβG showed greater resistance to product inhibition mediated by galactose as compared to it soluble counterpart and exhibited excellent catalytic activity even after its fourth successive reuse. The remarkable bioconversion rates of lactose from milk in batch reactors further revealed an attractive catalytic efficiency of β-galactosidase adsorbed on surface functionalized AgNPs thereby promoting its use in the production of lactose free dairy products. Crown Copyright © 2012 Published by Elsevier Ltd. All rights reserved.
AB - The present study demonstrates synthesis, characterization and surface functionalization of silver nanoparticles (AgNPs) via glutaraldehyde for high yield immobilization of Aspergillus oryzae β-galactosidase. Soluble β-galactosidase (SβG), enzyme adsorbed on unmodified AgNPs (UβG) and surface modified AgNPs (MβG) showed same pH-optima at pH 4.5. However, it was observed that MβG exhibited enhanced pH stability toward acidic and alkaline sides, and increased temperature resistance as compared to SβG and UβG. Michaelis constant, Km was increased nearly three-folds for MβG while Vmax for soluble and MβG was 0.515 mM/min and 0.495 mM/min, respectively. Furthermore, MβG showed greater resistance to product inhibition mediated by galactose as compared to it soluble counterpart and exhibited excellent catalytic activity even after its fourth successive reuse. The remarkable bioconversion rates of lactose from milk in batch reactors further revealed an attractive catalytic efficiency of β-galactosidase adsorbed on surface functionalized AgNPs thereby promoting its use in the production of lactose free dairy products. Crown Copyright © 2012 Published by Elsevier Ltd. All rights reserved.
UR - https://linkinghub.elsevier.com/retrieve/pii/S1359511312003716
UR - http://www.scopus.com/inward/record.url?scp=84870840125&partnerID=8YFLogxK
U2 - 10.1016/j.procbio.2012.10.002
DO - 10.1016/j.procbio.2012.10.002
M3 - Article
SN - 1359-5113
VL - 47
SP - 2427
EP - 2433
JO - Process Biochemistry
JF - Process Biochemistry
IS - 12
ER -