TY - JOUR
T1 - CRISPR-Based Diagnostics
T2 - Challenges and Potential Solutions toward Point-of-Care Applications
AU - Ghouneimy, Ahmed
AU - Mahas, Ahmed
AU - Marsic, Tin
AU - Aman, Rashid
AU - Mahfouz, Magdy
N1 - Funding Information:
This work was supported, in part, by the Smart Health Initiative at King Abdullah University of Science and Technology (KAUST) and Impact Acceleration Fund (IAF) and (Near Term Grand Challenge) NTGC grants from the KAUST (Innovation and Economic Development) IED to MM. All figures were created using Bioreneder.com .
Publisher Copyright:
© 2022 The Authors. Published by American Chemical Society.
PY - 2023/1/20
Y1 - 2023/1/20
N2 - The COVID-19 pandemic has challenged the conventional diagnostic field and revealed the need for decentralized Point of Care (POC) solutions. Although nucleic acid testing is considered to be the most sensitive and specific disease detection method, conventional testing platforms are expensive, confined to central laboratories, and are not deployable in low-resource settings. CRISPR-based diagnostics have emerged as promising tools capable of revolutionizing the field of molecular diagnostics. These platforms are inexpensive, simple, and do not require the use of special instrumentation, suggesting they could democratize access to disease diagnostics. However, there are several obstacles to the use of the current platforms for POC applications, including difficulties in sample processing and stability. In this review, we discuss key advancements in the field, with an emphasis on the challenges of sample processing, stability, multiplexing, amplification-free detection, signal interpretation, and process automation. We also discuss potential solutions for revolutionizing CRISPR-based diagnostics toward sample-to-answer diagnostic solutions for POC and home use.
AB - The COVID-19 pandemic has challenged the conventional diagnostic field and revealed the need for decentralized Point of Care (POC) solutions. Although nucleic acid testing is considered to be the most sensitive and specific disease detection method, conventional testing platforms are expensive, confined to central laboratories, and are not deployable in low-resource settings. CRISPR-based diagnostics have emerged as promising tools capable of revolutionizing the field of molecular diagnostics. These platforms are inexpensive, simple, and do not require the use of special instrumentation, suggesting they could democratize access to disease diagnostics. However, there are several obstacles to the use of the current platforms for POC applications, including difficulties in sample processing and stability. In this review, we discuss key advancements in the field, with an emphasis on the challenges of sample processing, stability, multiplexing, amplification-free detection, signal interpretation, and process automation. We also discuss potential solutions for revolutionizing CRISPR-based diagnostics toward sample-to-answer diagnostic solutions for POC and home use.
KW - biosensors
KW - COVID-19
KW - CRISPR
KW - CRISPR-Cas systems
KW - molecular diagnostics
KW - nucleic acid detection
KW - POC diagnostics
KW - SARS-CoV2
UR - http://www.scopus.com/inward/record.url?scp=85143970725&partnerID=8YFLogxK
U2 - 10.1021/acssynbio.2c00496
DO - 10.1021/acssynbio.2c00496
M3 - Review article
C2 - 36508352
AN - SCOPUS:85143970725
SN - 2161-5063
VL - 12
SP - 1
EP - 16
JO - ACS Synthetic Biology
JF - ACS Synthetic Biology
IS - 1
ER -