@article{ca930f69db5a4e6f98f8db26c48cebca,
title = "CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs",
abstract = "Genome editing using programmable nucleases has revolutionized biomedical research. CRISPR-Cas9 mediated zygote genome editing enables high efficient production of knockout animals suitable for studying development and relevant human diseases. Here we report efficient disabling pancreatogenesis in pig embryos via zygotic co-delivery of Cas9 mRNA and dual sgRNAs targeting the PDX1 gene, which when combined with chimeric-competent human pluriopotent stem cells may serve as a suitable platform for the xeno-generation of human tissues and organs in pigs.",
author = "Jun Wu and Marcela Vilarino and Keiichiro Suzuki and Daiji Okamura and Bogliotti, {Yanina Soledad} and Insung Park and Joan Rowe and Bret McNabb and Ross, {Pablo Juan} and Belmonte, {Juan Carlos Izpisua}",
note = "Funding Information: We thank Aaron Prinz, Kent Parker, and their crews for excellent assistance with embryo transfers and pig care at UC Davis. We would like to thank M. Schwarz, and P. Schwarz for administrative help. P.J.R. was supported by UC Davis Academic Senate New Research Grant. Work in the laboratory of JCIB was supported by the UCAM, G. Harold and Leila Y. Mathers Charitable Foundation and The Moxie Foundation. Publisher Copyright: {\textcopyright} 2017 The Author(s).",
year = "2017",
month = dec,
day = "1",
doi = "10.1038/s41598-017-08596-5",
language = "English (US)",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",
}
