TY - JOUR
T1 - Crystal Structure of Methylornithine Synthase (PylB): Insights into the Pyrrolysine Biosynthesis
AU - Quitterer, Felix
AU - List, Anja
AU - Eisenreich, Wolfgang
AU - Bacher, Adelbert
AU - Groll, Michael
N1 - KAUST Repository Item: Exported on 2020-10-01
Acknowledged KAUST grant number(s): FIC/2010/07
Acknowledgements: We thank Sophie Vieweg for experimental support and the staff of PXI of Paul Scherrer Institute, Swiss Light Source (Villigen, Switzerland) for help with data collection. This work was supported by the Hans-Fischer-Gesellschaft and by the King Abdullah University of Science and Technology (Award No. FIC/2010/07).
This publication acknowledges KAUST support, but has no KAUST affiliated authors.
PY - 2011/11/16
Y1 - 2011/11/16
N2 - Made by the barrel load: The biosynthetic pathway of the recently discovered 22nd amino acid, pyrrolysine, starts with an isomerization of lysine to methylornithine, catalyzed by PylB. The X-ray crystal structure of PylB is determined (see picture) and shows it has a TIM barrel fold. The sealed central cavity contains a [4Fe-4S] cluster, S-adenosylmethionine (SAM), and methylornithine, whose 2R,3R configuration could be confirmed. The data suggest a fragmentation-recombination mechanism via a glycyl radical intermediate. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
AB - Made by the barrel load: The biosynthetic pathway of the recently discovered 22nd amino acid, pyrrolysine, starts with an isomerization of lysine to methylornithine, catalyzed by PylB. The X-ray crystal structure of PylB is determined (see picture) and shows it has a TIM barrel fold. The sealed central cavity contains a [4Fe-4S] cluster, S-adenosylmethionine (SAM), and methylornithine, whose 2R,3R configuration could be confirmed. The data suggest a fragmentation-recombination mechanism via a glycyl radical intermediate. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
UR - http://hdl.handle.net/10754/597905
UR - http://doi.wiley.com/10.1002/anie.201106765
UR - http://www.scopus.com/inward/record.url?scp=84856471938&partnerID=8YFLogxK
U2 - 10.1002/anie.201106765
DO - 10.1002/anie.201106765
M3 - Article
C2 - 22095926
SN - 1433-7851
VL - 51
SP - 1339
EP - 1342
JO - Angewandte Chemie International Edition
JF - Angewandte Chemie International Edition
IS - 6
ER -