TY - JOUR
T1 - Cyclic mononucleotides modulate potassium and calcium flux responses to H2O2 in Arabidopsis roots
AU - Ordoñez, Natalia Maria
AU - Marondedze, Claudius
AU - Thomas, Ludivine
AU - Pasqualini, Stefania
AU - Shabala, Lana
AU - Shabala, Sergey
AU - Gehring, Christoph A
N1 - KAUST Repository Item: Exported on 2020-10-01
PY - 2014/2/13
Y1 - 2014/2/13
N2 - Cyclic mononucleotides are messengers in plant stress responses. Here we show that hydrogen peroxide (H2O2) induces rapid net K+-efflux and Ca2+-influx in Arabidopsis roots. Pre-treatment with either 10 μM cAMP or cGMP for 1 or 24 h does significantly reduce net K+-leakage and Ca2+-influx, and in the case of the K+-fluxes, the cell permeant cyclic mononucleotides are more effective. We also examined the effect of 10 μM of the cell permeant 8-Br-cGMP on the Arabidopsis microsomal proteome and noted a specific increase in proteins with a role in stress responses and ion transport, suggesting that cGMP is sufficient to directly and/or indirectly induce complex adaptive changes to cellular stresses induced by H2O2. © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
AB - Cyclic mononucleotides are messengers in plant stress responses. Here we show that hydrogen peroxide (H2O2) induces rapid net K+-efflux and Ca2+-influx in Arabidopsis roots. Pre-treatment with either 10 μM cAMP or cGMP for 1 or 24 h does significantly reduce net K+-leakage and Ca2+-influx, and in the case of the K+-fluxes, the cell permeant cyclic mononucleotides are more effective. We also examined the effect of 10 μM of the cell permeant 8-Br-cGMP on the Arabidopsis microsomal proteome and noted a specific increase in proteins with a role in stress responses and ion transport, suggesting that cGMP is sufficient to directly and/or indirectly induce complex adaptive changes to cellular stresses induced by H2O2. © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
UR - http://hdl.handle.net/10754/552388
UR - http://linkinghub.elsevier.com/retrieve/pii/S0014579314001057
UR - http://www.scopus.com/inward/record.url?scp=84896096423&partnerID=8YFLogxK
U2 - 10.1016/j.febslet.2014.01.062
DO - 10.1016/j.febslet.2014.01.062
M3 - Article
C2 - 24530500
SN - 0014-5793
VL - 588
SP - 1008
EP - 1015
JO - FEBS Letters
JF - FEBS Letters
IS - 6
ER -