TY - GEN
T1 - Design of a scalable DNA shearing system using phased-array ultrasonic transducer
AU - Dev, Kapil
AU - Sharma, Smriti
AU - Vivek, Vibhu
AU - Hadimioglu, Babur
AU - Massoud, Yehia
N1 - Generated from Scopus record by KAUST IRTS on 2022-09-13
PY - 2011/12/1
Y1 - 2011/12/1
N2 - In this paper, we present the design of a Deoxyribonucleic Acid (DNA) shearing system based on unique acoustic waves generated using a phased-array transducer. Four 90° sector-transducers are used to build a circular array transducer. Each 90°-transducer is excited with separate RF signal of same or different phase. The proposed transducer structure generates bulk lateral ultrasonic waves in the DNA sample; the lateral waves produce both convergence and vortexing effects in the sample. The converged lateral acoustic waves are required to break the DNA sample-meniscus inside the tube and the rotational component of acoustic field is used to recirculate the DNA sample to get homogeneous shearing with tight fragment distribution. Finally, we present the experimental results of DNA sheared to different mean fragment sizes using the proposed system and validate the shearing-capability of the proposed system. © 2011 IEEE.
AB - In this paper, we present the design of a Deoxyribonucleic Acid (DNA) shearing system based on unique acoustic waves generated using a phased-array transducer. Four 90° sector-transducers are used to build a circular array transducer. Each 90°-transducer is excited with separate RF signal of same or different phase. The proposed transducer structure generates bulk lateral ultrasonic waves in the DNA sample; the lateral waves produce both convergence and vortexing effects in the sample. The converged lateral acoustic waves are required to break the DNA sample-meniscus inside the tube and the rotational component of acoustic field is used to recirculate the DNA sample to get homogeneous shearing with tight fragment distribution. Finally, we present the experimental results of DNA sheared to different mean fragment sizes using the proposed system and validate the shearing-capability of the proposed system. © 2011 IEEE.
UR - http://ieeexplore.ieee.org/document/6122231/
UR - http://www.scopus.com/inward/record.url?scp=84863410332&partnerID=8YFLogxK
U2 - 10.1109/ICECS.2011.6122231
DO - 10.1109/ICECS.2011.6122231
M3 - Conference contribution
SN - 9781457718458
SP - 129
EP - 132
BT - 2011 18th IEEE International Conference on Electronics, Circuits, and Systems, ICECS 2011
ER -