Abstract
Methods for determining the viability and infectivity of Cryptosporidium oocysts after inactivation by disinfectants, have received much attention. In this paper, five commonly used methods, namely animal infectivity, cell culture, fluorogenic vital dyes, in vitro excystation and reverse transcription polymerase chain reaction (RT-PCR), are reviewed to provide useful information for choosing and optimizing the detection methods. The research process, applicability, reliability, and advantages and disadvantages are systematically discussed.
Original language | English (US) |
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Pages (from-to) | 449-455 |
Number of pages | 7 |
Journal | Huanjing Kexue Xuebao/Acta Scientiae Circumstantiae |
Volume | 30 |
Issue number | 3 |
State | Published - Mar 2010 |
Externally published | Yes |
Keywords
- Animal infectivity
- Cell culture
- Cryptosporidium
- Fluorogenic vital dyes
- In vitro excystation
- Infectivity
- RT-PCR
- Viability
ASJC Scopus subject areas
- Environmental Engineering
- Environmental Chemistry
- General Environmental Science