Determination of surface concentrations of individual molecule-layers used in nanoscale biosensors by in situ ATR-FTIR spectroscopy

Manuel Punzet; Dieter Baurecht; Franz Varga; Heidrun Karlic; Clemens Heitzinger

doi:10.1039/c2nr12038k

Determination of surface concentrations of individual molecule-layers used in nanoscale biosensors by in situ ATR-FTIR spectroscopy

Manuel Punzet, Dieter Baurecht, Franz Varga, Heidrun Karlic, Clemens Heitzinger

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

For the development of nanowire sensors for chemical and medical detection purposes, the optimal functionalization of the surface is a mandatory component. Quantitative ATR-FTIR spectroscopy was used in situ to investigate the step-by-step layer formation of typical functionalization protocols and to determine the respective molecule surface concentrations. BSA, anti-TNF-α and anti-PSA antibodies were bound via 3-(trimethoxy)butylsilyl aldehyde linkers to silicon-oxide surfaces in order to investigate surface functionalization of nanowires. Maximum determined surface concentrations were 7.17 × 10 -13 mol cm -2 for BSA, 1.7 × 10 -13 mol cm -2 for anti-TNF-α antibody, 6.1 × 10 -13 mol cm -2 for anti-PSA antibody, 3.88 × 10 -13 mol cm -2 for TNF-α and 7.0 × 10 -13 mol cm -2 for PSA. Furthermore we performed antibody-antigen binding experiments and determined the specific binding ratios. The maximum possible ratio of 2 was obtained at bulk concentrations of the antigen in the μg ml -1 range for TNF-α and PSA. © 2012 The Royal Society of Chemistry.

Original language	English (US)
Pages (from-to)	2431
Journal	Nanoscale
Volume	4
Issue number	7
DOIs	https://doi.org/10.1039/c2nr12038k
State	Published - 2012
Externally published	Yes

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@article{daa1c954be534c848728a8e5cacaf831,

title = "Determination of surface concentrations of individual molecule-layers used in nanoscale biosensors by in situ ATR-FTIR spectroscopy",

abstract = "For the development of nanowire sensors for chemical and medical detection purposes, the optimal functionalization of the surface is a mandatory component. Quantitative ATR-FTIR spectroscopy was used in situ to investigate the step-by-step layer formation of typical functionalization protocols and to determine the respective molecule surface concentrations. BSA, anti-TNF-α and anti-PSA antibodies were bound via 3-(trimethoxy)butylsilyl aldehyde linkers to silicon-oxide surfaces in order to investigate surface functionalization of nanowires. Maximum determined surface concentrations were 7.17 × 10 -13 mol cm -2 for BSA, 1.7 × 10 -13 mol cm -2 for anti-TNF-α antibody, 6.1 × 10 -13 mol cm -2 for anti-PSA antibody, 3.88 × 10 -13 mol cm -2 for TNF-α and 7.0 × 10 -13 mol cm -2 for PSA. Furthermore we performed antibody-antigen binding experiments and determined the specific binding ratios. The maximum possible ratio of 2 was obtained at bulk concentrations of the antigen in the μg ml -1 range for TNF-α and PSA. {\textcopyright} 2012 The Royal Society of Chemistry.",

author = "Manuel Punzet and Dieter Baurecht and Franz Varga and Heidrun Karlic and Clemens Heitzinger",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledged KAUST grant number(s): KUK-I1-007-43 Acknowledgements: We thank Prof. Wolfgang Lindner of the Chemical Faculty of the University of Vienna for his assistance in silane chemistry and the facilitation of the silanization protocol and Prof. Falkenhagen of the Danube University, Krems (Austria) for providing us with monoclonal hTNF-alpha antibody. This work has been financially supported by Austrian Science Fund (FWF) Project No. P20871-N31 and by Award No. KUK-I1-007-43, made by the King Abdullah University of Science and Technology (KAUST). We dedicate this article to the memory of Prof. Urs Peter Fringeli. This publication acknowledges KAUST support, but has no KAUST affiliated authors.",

year = "2012",

doi = "10.1039/c2nr12038k",

language = "English (US)",

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pages = "2431",

journal = "Nanoscale",

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T1 - Determination of surface concentrations of individual molecule-layers used in nanoscale biosensors by in situ ATR-FTIR spectroscopy

AU - Punzet, Manuel

AU - Baurecht, Dieter

AU - Varga, Franz

AU - Karlic, Heidrun

AU - Heitzinger, Clemens

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledged KAUST grant number(s): KUK-I1-007-43 Acknowledgements: We thank Prof. Wolfgang Lindner of the Chemical Faculty of the University of Vienna for his assistance in silane chemistry and the facilitation of the silanization protocol and Prof. Falkenhagen of the Danube University, Krems (Austria) for providing us with monoclonal hTNF-alpha antibody. This work has been financially supported by Austrian Science Fund (FWF) Project No. P20871-N31 and by Award No. KUK-I1-007-43, made by the King Abdullah University of Science and Technology (KAUST). We dedicate this article to the memory of Prof. Urs Peter Fringeli. This publication acknowledges KAUST support, but has no KAUST affiliated authors.

PY - 2012

Y1 - 2012

N2 - For the development of nanowire sensors for chemical and medical detection purposes, the optimal functionalization of the surface is a mandatory component. Quantitative ATR-FTIR spectroscopy was used in situ to investigate the step-by-step layer formation of typical functionalization protocols and to determine the respective molecule surface concentrations. BSA, anti-TNF-α and anti-PSA antibodies were bound via 3-(trimethoxy)butylsilyl aldehyde linkers to silicon-oxide surfaces in order to investigate surface functionalization of nanowires. Maximum determined surface concentrations were 7.17 × 10 -13 mol cm -2 for BSA, 1.7 × 10 -13 mol cm -2 for anti-TNF-α antibody, 6.1 × 10 -13 mol cm -2 for anti-PSA antibody, 3.88 × 10 -13 mol cm -2 for TNF-α and 7.0 × 10 -13 mol cm -2 for PSA. Furthermore we performed antibody-antigen binding experiments and determined the specific binding ratios. The maximum possible ratio of 2 was obtained at bulk concentrations of the antigen in the μg ml -1 range for TNF-α and PSA. © 2012 The Royal Society of Chemistry.

AB - For the development of nanowire sensors for chemical and medical detection purposes, the optimal functionalization of the surface is a mandatory component. Quantitative ATR-FTIR spectroscopy was used in situ to investigate the step-by-step layer formation of typical functionalization protocols and to determine the respective molecule surface concentrations. BSA, anti-TNF-α and anti-PSA antibodies were bound via 3-(trimethoxy)butylsilyl aldehyde linkers to silicon-oxide surfaces in order to investigate surface functionalization of nanowires. Maximum determined surface concentrations were 7.17 × 10 -13 mol cm -2 for BSA, 1.7 × 10 -13 mol cm -2 for anti-TNF-α antibody, 6.1 × 10 -13 mol cm -2 for anti-PSA antibody, 3.88 × 10 -13 mol cm -2 for TNF-α and 7.0 × 10 -13 mol cm -2 for PSA. Furthermore we performed antibody-antigen binding experiments and determined the specific binding ratios. The maximum possible ratio of 2 was obtained at bulk concentrations of the antigen in the μg ml -1 range for TNF-α and PSA. © 2012 The Royal Society of Chemistry.

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DO - 10.1039/c2nr12038k

M3 - Article

C2 - 22399200

SN - 2040-3364

VL - 4

SP - 2431

JO - Nanoscale

JF - Nanoscale

IS - 7

ER -

Determination of surface concentrations of individual molecule-layers used in nanoscale biosensors by in situ ATR-FTIR spectroscopy

Abstract

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