Development and Application of Classical Genetics in Toxoplasma gondii

James W. Ajioka; L. David Sibley

doi:10.1016/B978-0-12-396481-6.00016-7

Development and Application of Classical Genetics in Toxoplasma gondii

James W. Ajioka, L. David Sibley

Research output: Chapter in Book/Report/Conference proceeding › Chapter

2 Scopus citations

Abstract

Toxoplasma gondii undergoes its sexual phase within the feline intestine. The sexual stages have been an important tool for understanding the biology of the parasite using forward genetics approaches. Genetic crosses were performed by feeding cats bradyzoites from T. gondii strains that differ in virulence and drug resistance. Using progeny from these crosses, the genes responsible for virulence and other biologically important phenotypes have been mapped using classical forward genetic techniques. This chapter reviews the principles of forward genetics in T. gondii and illustrates how the classical genetics approach enabled identification of Toxoplasma virulence genes including the ROP family of rhoptry kinases and pseudokinases. © 2014 Elsevier Ltd. All rights reserved.

Original language	English (US)
Title of host publication	Toxoplasma Gondii
Publisher	Elsevier
Pages	551-576
Number of pages	26
ISBN (Print)	9780123964816
DOIs	https://doi.org/10.1016/B978-0-12-396481-6.00016-7
State	Published - 2014
Externally published	Yes

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10.1016/B978-0-12-396481-6.00016-7

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title = "Development and Application of Classical Genetics in Toxoplasma gondii",

abstract = "Toxoplasma gondii undergoes its sexual phase within the feline intestine. The sexual stages have been an important tool for understanding the biology of the parasite using forward genetics approaches. Genetic crosses were performed by feeding cats bradyzoites from T. gondii strains that differ in virulence and drug resistance. Using progeny from these crosses, the genes responsible for virulence and other biologically important phenotypes have been mapped using classical forward genetic techniques. This chapter reviews the principles of forward genetics in T. gondii and illustrates how the classical genetics approach enabled identification of Toxoplasma virulence genes including the ROP family of rhoptry kinases and pseudokinases. {\textcopyright} 2014 Elsevier Ltd. All rights reserved.",

author = "Ajioka, {James W.} and Sibley, {L. David}",

note = "KAUST Repository Item: Exported on 2021-07-01 Acknowledgements: The National Institutes of Health (USA), United States Department of Agriculture (USA), Burroughs Wellcome Fund (USA), American Heart Association (USA), Wellcome Trust (UK), King Abdulla University of Science and Technology (KAUST), and Biotechnology and Biological Sciences Research Council (UK) have supported work in the authors{\textquoteright} laboratories. This publication acknowledges KAUST support, but has no KAUST affiliated authors.",

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doi = "10.1016/B978-0-12-396481-6.00016-7",

language = "English (US)",

isbn = "9780123964816",

pages = "551--576",

booktitle = "Toxoplasma Gondii",

publisher = "Elsevier",

address = "Netherlands",

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T1 - Development and Application of Classical Genetics in Toxoplasma gondii

AU - Ajioka, James W.

AU - Sibley, L. David

N1 - KAUST Repository Item: Exported on 2021-07-01 Acknowledgements: The National Institutes of Health (USA), United States Department of Agriculture (USA), Burroughs Wellcome Fund (USA), American Heart Association (USA), Wellcome Trust (UK), King Abdulla University of Science and Technology (KAUST), and Biotechnology and Biological Sciences Research Council (UK) have supported work in the authors’ laboratories. This publication acknowledges KAUST support, but has no KAUST affiliated authors.

PY - 2014

Y1 - 2014

N2 - Toxoplasma gondii undergoes its sexual phase within the feline intestine. The sexual stages have been an important tool for understanding the biology of the parasite using forward genetics approaches. Genetic crosses were performed by feeding cats bradyzoites from T. gondii strains that differ in virulence and drug resistance. Using progeny from these crosses, the genes responsible for virulence and other biologically important phenotypes have been mapped using classical forward genetic techniques. This chapter reviews the principles of forward genetics in T. gondii and illustrates how the classical genetics approach enabled identification of Toxoplasma virulence genes including the ROP family of rhoptry kinases and pseudokinases. © 2014 Elsevier Ltd. All rights reserved.

AB - Toxoplasma gondii undergoes its sexual phase within the feline intestine. The sexual stages have been an important tool for understanding the biology of the parasite using forward genetics approaches. Genetic crosses were performed by feeding cats bradyzoites from T. gondii strains that differ in virulence and drug resistance. Using progeny from these crosses, the genes responsible for virulence and other biologically important phenotypes have been mapped using classical forward genetic techniques. This chapter reviews the principles of forward genetics in T. gondii and illustrates how the classical genetics approach enabled identification of Toxoplasma virulence genes including the ROP family of rhoptry kinases and pseudokinases. © 2014 Elsevier Ltd. All rights reserved.

UR - http://hdl.handle.net/10754/669840

UR - https://linkinghub.elsevier.com/retrieve/pii/B9780123964816000167

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U2 - 10.1016/B978-0-12-396481-6.00016-7

DO - 10.1016/B978-0-12-396481-6.00016-7

M3 - Chapter

SN - 9780123964816

SP - 551

EP - 576

BT - Toxoplasma Gondii

PB - Elsevier

ER -

Development and Application of Classical Genetics in Toxoplasma gondii

Abstract

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