Development and characterization of guar gum nanoparticles for oral immunization against tuberculosis

Mandeep Kaur; Basant Malik; Tarun Garg; Goutam Rath; Amit K. Goyal

doi:10.3109/10717544.2014.894594

Development and characterization of guar gum nanoparticles for oral immunization against tuberculosis

Mandeep Kaur, Basant Malik, Tarun Garg, Goutam Rath, Amit K. Goyal

Research output: Contribution to journal › Article › peer-review

69 Scopus citations

Abstract

The main aim of this study was to develop an effective carrier system containing Ag85A-loaded guar gum nanoparticles for oral vaccination against tuberculosis. Nanoparticles were prepared by Nanoprecipitation method. The developed particles with mean diameter 895.5±14.73nm and high antigen entrapment seem to be optimum for oral vaccine delivery. The acid protection assay, Peyer's patch uptake study and in-vitro antigen study confirmed that the developed formulations can protect the antigen from harsh gastric environment and can safely deliver the antigen to the intestinal region. In vivo studies data indicated that the developed nanocarriers can induce a strong mucosal as well as systemic immune response. Therefore, the experimental evidence suggests that guar-gum nanoparticle findings indicated that the guar gum nanoparticles can be utilized for safe and effective vaccine delivery via oral route.

Original language	English (US)
Pages (from-to)	328-334
Number of pages	7
Journal	Drug Delivery
Volume	22
Issue number	3
DOIs	https://doi.org/10.3109/10717544.2014.894594
State	Published - May 1 2015
Externally published	Yes

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10.3109/10717544.2014.894594

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title = "Development and characterization of guar gum nanoparticles for oral immunization against tuberculosis",

abstract = "The main aim of this study was to develop an effective carrier system containing Ag85A-loaded guar gum nanoparticles for oral vaccination against tuberculosis. Nanoparticles were prepared by Nanoprecipitation method. The developed particles with mean diameter 895.5±14.73nm and high antigen entrapment seem to be optimum for oral vaccine delivery. The acid protection assay, Peyer's patch uptake study and in-vitro antigen study confirmed that the developed formulations can protect the antigen from harsh gastric environment and can safely deliver the antigen to the intestinal region. In vivo studies data indicated that the developed nanocarriers can induce a strong mucosal as well as systemic immune response. Therefore, the experimental evidence suggests that guar-gum nanoparticle findings indicated that the guar gum nanoparticles can be utilized for safe and effective vaccine delivery via oral route.",

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AU - Kaur, Mandeep

AU - Malik, Basant

AU - Garg, Tarun

AU - Rath, Goutam

AU - Goyal, Amit K.

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PY - 2015/5/1

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AB - The main aim of this study was to develop an effective carrier system containing Ag85A-loaded guar gum nanoparticles for oral vaccination against tuberculosis. Nanoparticles were prepared by Nanoprecipitation method. The developed particles with mean diameter 895.5±14.73nm and high antigen entrapment seem to be optimum for oral vaccine delivery. The acid protection assay, Peyer's patch uptake study and in-vitro antigen study confirmed that the developed formulations can protect the antigen from harsh gastric environment and can safely deliver the antigen to the intestinal region. In vivo studies data indicated that the developed nanocarriers can induce a strong mucosal as well as systemic immune response. Therefore, the experimental evidence suggests that guar-gum nanoparticle findings indicated that the guar gum nanoparticles can be utilized for safe and effective vaccine delivery via oral route.

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JO - Drug Delivery

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Development and characterization of guar gum nanoparticles for oral immunization against tuberculosis

Abstract

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