TY - JOUR
T1 - Development of 3D PVA scaffolds for cardiac tissue engineering and cell screening applications
AU - Dattola, Elisabetta
AU - Parrotta, Elvira Immacolata
AU - Scalise, Stefania
AU - Perozziello, Gerardo
AU - Limongi, Tania
AU - Candeloro, Patrizio
AU - Coluccio, Maria Laura
AU - Maletta, Carmine
AU - Bruno, Luigi
AU - De Angelis, Maria Teresa
AU - Santamaria, Gianluca
AU - Mollace, Vincenzo
AU - Lamanna, Ernesto
AU - Di Fabrizio, Enzo M.
AU - Cuda, Giovanni
N1 - KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: This work was supported by the project for Young researchers financed from the Italian Ministry of Health “High throughput analysis of cancer cells for therapy evaluation by microfluidic platforms integrating plasmonic nanodevices” (CUP J65C13001350001, project No. GR-2010-2311677) granted to the nanotechnology laboratory of the Department of Experimental and Clinical Medicine of the University “Magna Graecia” of Catanzaro.
PY - 2019
Y1 - 2019
N2 - The aim of this study was the design of a 3D scaffold composed of poly(vinyl) alcohol (PVA) for cardiac tissue engineering (CTE) applications. The PVA scaffold was fabricated using a combination of gas foaming and freeze-drying processes that did not need any cross-linking agents. We obtained a biocompatible porous matrix with excellent mechanical properties. We measured the stress–strain curves of the PVA scaffolds and we showed that the elastic behavior is similar to that of the extracellular matrix of muscles. The SEM observations revealed that the scaffolds possess micro pores having diameters ranging from 10 μm to 370 μm that fit to the dimensions of the cells. A further purpose of this study was to test scaffolds ability to support human induced pluripotent stem cells growth and differentiation into cardiomyocytes. As the proliferation tests show, the number of live stem cells on the scaffold after 12 days was increased with respect to the initial number of cells, revealing the cytocompatibility of the substrate. In addition, the differentiated cells on the PVA scaffolds expressed anti-troponin T, a marker specific of the cardiac sarcomere. We demonstrated the ability of the cardiomyocytes to pulse within the scaffolds. In conclusion, the developed scaffold show the potential to be used as a biomaterial for CTE applications.
AB - The aim of this study was the design of a 3D scaffold composed of poly(vinyl) alcohol (PVA) for cardiac tissue engineering (CTE) applications. The PVA scaffold was fabricated using a combination of gas foaming and freeze-drying processes that did not need any cross-linking agents. We obtained a biocompatible porous matrix with excellent mechanical properties. We measured the stress–strain curves of the PVA scaffolds and we showed that the elastic behavior is similar to that of the extracellular matrix of muscles. The SEM observations revealed that the scaffolds possess micro pores having diameters ranging from 10 μm to 370 μm that fit to the dimensions of the cells. A further purpose of this study was to test scaffolds ability to support human induced pluripotent stem cells growth and differentiation into cardiomyocytes. As the proliferation tests show, the number of live stem cells on the scaffold after 12 days was increased with respect to the initial number of cells, revealing the cytocompatibility of the substrate. In addition, the differentiated cells on the PVA scaffolds expressed anti-troponin T, a marker specific of the cardiac sarcomere. We demonstrated the ability of the cardiomyocytes to pulse within the scaffolds. In conclusion, the developed scaffold show the potential to be used as a biomaterial for CTE applications.
UR - http://hdl.handle.net/10754/631740
UR - https://pubs.rsc.org/en/Content/ArticleLanding/2019/RA/C8RA08187E#!divAbstract
UR - http://www.scopus.com/inward/record.url?scp=85061939719&partnerID=8YFLogxK
U2 - 10.1039/c8ra08187e
DO - 10.1039/c8ra08187e
M3 - Article
SN - 2046-2069
VL - 9
SP - 4246
EP - 4257
JO - RSC Advances
JF - RSC Advances
IS - 8
ER -