Development of oral CTL vaccine using a CTP-integrated Sabin 1 poliovirus-based vector system

Seung Soo Han; Jinjoo Lee; Yideul Jung; Myeong Ho Kang; Jung Hyub Hong; Min Suk Cha; Yu Jin Park; Ezra Lee; Cheol Hee Yoon; Yong Soo Bae

doi:10.1016/j.vaccine.2015.07.072

Development of oral CTL vaccine using a CTP-integrated Sabin 1 poliovirus-based vector system

Seung Soo Han, Jinjoo Lee, Yideul Jung, Myeong Ho Kang, Jung Hyub Hong, Min Suk Cha, Yu Jin Park, Ezra Lee, Cheol Hee Yoon, Yong Soo Bae^*

^*Corresponding author for this work

Physical Sciences and Engineering

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

We developed a CTL vaccine vector by modification of the RPS-Vax system, a mucosal vaccine vector derived from a poliovirus Sabin 1 strain, and generated an oral CTL vaccine against HIV-1. A DNA fragment encoding a cytoplasmic transduction peptide (CTP) was integrated into the RPS-Vax system to generate RPS-CTP, a CTL vaccine vector. An HIV-1 p24 cDNA fragment was introduced into the RPS-CTP vector system and a recombinant poliovirus (rec-PV) named vRPS-CTP/p24 was produced. vRPS-CTP/p24 was genetically stable and efficiently induced Th1 immunity and p24-specific CTLs in immunized poliovirus receptor-transgenic (PVR-Tg) mice. In challenge experiments, PVR-Tg mice that were pre-immunized orally with vRPS-CTP/p24 were resistant to challenge with a lethal dose of p24-expressing recombinant vaccinia virus (rMVA-p24). These results suggested that the RPS-CTP vector system had potential for developing oral CTL vaccines against infectious diseases.

Original language	English (US)
Article number	16768
Pages (from-to)	4827-4836
Number of pages	10
Journal	Vaccine
Volume	33
Issue number	38
DOIs	https://doi.org/10.1016/j.vaccine.2015.07.072
State	Published - Sep 11 2015

Keywords

HIV-1 p24
Oral CTL vaccine
P24-specific CTL
Poliovirus Sabin 1 vector
RPS-CTP CTL vector
Recombinant poliovirus

ASJC Scopus subject areas

Public Health, Environmental and Occupational Health
General Immunology and Microbiology
Infectious Diseases
Molecular Medicine
General Veterinary

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.vaccine.2015.07.072

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title = "Development of oral CTL vaccine using a CTP-integrated Sabin 1 poliovirus-based vector system",

abstract = "We developed a CTL vaccine vector by modification of the RPS-Vax system, a mucosal vaccine vector derived from a poliovirus Sabin 1 strain, and generated an oral CTL vaccine against HIV-1. A DNA fragment encoding a cytoplasmic transduction peptide (CTP) was integrated into the RPS-Vax system to generate RPS-CTP, a CTL vaccine vector. An HIV-1 p24 cDNA fragment was introduced into the RPS-CTP vector system and a recombinant poliovirus (rec-PV) named vRPS-CTP/p24 was produced. vRPS-CTP/p24 was genetically stable and efficiently induced Th1 immunity and p24-specific CTLs in immunized poliovirus receptor-transgenic (PVR-Tg) mice. In challenge experiments, PVR-Tg mice that were pre-immunized orally with vRPS-CTP/p24 were resistant to challenge with a lethal dose of p24-expressing recombinant vaccinia virus (rMVA-p24). These results suggested that the RPS-CTP vector system had potential for developing oral CTL vaccines against infectious diseases.",

keywords = "HIV-1 p24, Oral CTL vaccine, P24-specific CTL, Poliovirus Sabin 1 vector, RPS-CTP CTL vector, Recombinant poliovirus",

author = "Han, {Seung Soo} and Jinjoo Lee and Yideul Jung and Kang, {Myeong Ho} and Hong, {Jung Hyub} and Cha, {Min Suk} and Park, {Yu Jin} and Ezra Lee and Yoon, {Cheol Hee} and Bae, {Yong Soo}",

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year = "2015",

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doi = "10.1016/j.vaccine.2015.07.072",

language = "English (US)",

volume = "33",

pages = "4827--4836",

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T1 - Development of oral CTL vaccine using a CTP-integrated Sabin 1 poliovirus-based vector system

AU - Han, Seung Soo

AU - Lee, Jinjoo

AU - Jung, Yideul

AU - Kang, Myeong Ho

AU - Hong, Jung Hyub

AU - Cha, Min Suk

AU - Park, Yu Jin

AU - Lee, Ezra

AU - Yoon, Cheol Hee

AU - Bae, Yong Soo

PY - 2015/9/11

Y1 - 2015/9/11

N2 - We developed a CTL vaccine vector by modification of the RPS-Vax system, a mucosal vaccine vector derived from a poliovirus Sabin 1 strain, and generated an oral CTL vaccine against HIV-1. A DNA fragment encoding a cytoplasmic transduction peptide (CTP) was integrated into the RPS-Vax system to generate RPS-CTP, a CTL vaccine vector. An HIV-1 p24 cDNA fragment was introduced into the RPS-CTP vector system and a recombinant poliovirus (rec-PV) named vRPS-CTP/p24 was produced. vRPS-CTP/p24 was genetically stable and efficiently induced Th1 immunity and p24-specific CTLs in immunized poliovirus receptor-transgenic (PVR-Tg) mice. In challenge experiments, PVR-Tg mice that were pre-immunized orally with vRPS-CTP/p24 were resistant to challenge with a lethal dose of p24-expressing recombinant vaccinia virus (rMVA-p24). These results suggested that the RPS-CTP vector system had potential for developing oral CTL vaccines against infectious diseases.

AB - We developed a CTL vaccine vector by modification of the RPS-Vax system, a mucosal vaccine vector derived from a poliovirus Sabin 1 strain, and generated an oral CTL vaccine against HIV-1. A DNA fragment encoding a cytoplasmic transduction peptide (CTP) was integrated into the RPS-Vax system to generate RPS-CTP, a CTL vaccine vector. An HIV-1 p24 cDNA fragment was introduced into the RPS-CTP vector system and a recombinant poliovirus (rec-PV) named vRPS-CTP/p24 was produced. vRPS-CTP/p24 was genetically stable and efficiently induced Th1 immunity and p24-specific CTLs in immunized poliovirus receptor-transgenic (PVR-Tg) mice. In challenge experiments, PVR-Tg mice that were pre-immunized orally with vRPS-CTP/p24 were resistant to challenge with a lethal dose of p24-expressing recombinant vaccinia virus (rMVA-p24). These results suggested that the RPS-CTP vector system had potential for developing oral CTL vaccines against infectious diseases.

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KW - P24-specific CTL

KW - Poliovirus Sabin 1 vector

KW - RPS-CTP CTL vector

KW - Recombinant poliovirus

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Development of oral CTL vaccine using a CTP-integrated Sabin 1 poliovirus-based vector system

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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