Developmental expression of chick Twist and its regulation during limb patterning

A. T. Tavares, J. C. Izpisúa-Belmonte, J. Rodríguez-León*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

48 Scopus citations


We have isolated a chick Twist gene (cTwist) and examined its expression pattern during development by whole mount in situ hybridization. In early embryos, cTwist transcripts are found in the developing somites, lateral plate mesoderm, limb mesenchyme, branchial arches and head mesenchyme. At later stages, cTwist expression is found in the sclerotome and dermatome, limb bud mesenchyme, interdigital regions, and distal mesenchyme of the maxillary and mandibular processes. In the developing feathers, cTwist is expressed in the mesenchyme of the buds and becomes restricted to the proximal region of the feather filaments. Additionally, we report that the expression of cTwist in the limb mesenchyme is regulated by the AER, FGFs, RA and SHH. The FGFs secreted by the AER seem to have a critical role in maintaining cTwist expression. SHH is also able to maintain cTwist expression but only in the presence of the AER. Overall, our results provide new evidence that reinforce the existence of an interplay between the cTwist and FGF signalling pathways.

Original languageEnglish (US)
Pages (from-to)707-713
Number of pages7
JournalInternational Journal of Developmental Biology
Issue number5-6
StatePublished - 2001
Externally publishedYes


  • Chick embryo
  • FGF
  • Limb development
  • SHH
  • Twist

ASJC Scopus subject areas

  • Embryology
  • Developmental Biology


Dive into the research topics of 'Developmental expression of chick Twist and its regulation during limb patterning'. Together they form a unique fingerprint.

Cite this