Differential Cotton leaf crumple virus-VIGS-mediated gene silencing and viral genome localization in different Gossypium hirsutum genetic backgrounds

Ali Idris; John Richard Tuttle; Dominique Niki Robertson; Candace H. Haigler; Judith K. Brown

doi:10.1016/j.pmpp.2010.07.002

Differential Cotton leaf crumple virus-VIGS-mediated gene silencing and viral genome localization in different Gossypium hirsutum genetic backgrounds

Ali Idris, John Richard Tuttle, Dominique Niki Robertson, Candace H. Haigler, Judith K. Brown

King Abdullah University of Science and Technology

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

A Cotton leaf crumple virus (CLCrV)-based gene silencing vector containing a fragment of the Gossypium hirsutum Magnesium chelatase subunit I was used to establish endogenous gene silencing in cotton of varied genetic backgrounds. Biolistic inoculation resulted in systemic and persistent photo-bleaching of the leaves and bolls of the seven cultivars tested, however, the intensity of silencing was variable. CLCrV-VIGS-mediated expression of green fluorescent protein was used to monitor the in planta distribution of the vector, indicating successful phloem invasion in all cultivars tested. Acala SJ-1, one of the cotton cultivars, was identified as a particularly optimal candidate for CLCrV-VIGS-based cotton reverse-genetics. © 2010 Elsevier Ltd.

Original language	English (US)
Pages (from-to)	13-22
Number of pages	10
Journal	Physiological and Molecular Plant Pathology
Volume	75
Issue number	1-2
DOIs	https://doi.org/10.1016/j.pmpp.2010.07.002
State	Published - Aug 4 2010

ASJC Scopus subject areas

Plant Science
Genetics

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10.1016/j.pmpp.2010.07.002

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title = "Differential Cotton leaf crumple virus-VIGS-mediated gene silencing and viral genome localization in different Gossypium hirsutum genetic backgrounds",

abstract = "A Cotton leaf crumple virus (CLCrV)-based gene silencing vector containing a fragment of the Gossypium hirsutum Magnesium chelatase subunit I was used to establish endogenous gene silencing in cotton of varied genetic backgrounds. Biolistic inoculation resulted in systemic and persistent photo-bleaching of the leaves and bolls of the seven cultivars tested, however, the intensity of silencing was variable. CLCrV-VIGS-mediated expression of green fluorescent protein was used to monitor the in planta distribution of the vector, indicating successful phloem invasion in all cultivars tested. Acala SJ-1, one of the cotton cultivars, was identified as a particularly optimal candidate for CLCrV-VIGS-based cotton reverse-genetics. {\textcopyright} 2010 Elsevier Ltd.",

author = "Ali Idris and Tuttle, {John Richard} and Robertson, {Dominique Niki} and Haigler, {Candace H.} and Brown, {Judith K.}",

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year = "2010",

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doi = "10.1016/j.pmpp.2010.07.002",

language = "English (US)",

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journal = "Physiological and Molecular Plant Pathology",

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Differential Cotton leaf crumple virus-VIGS-mediated gene silencing and viral genome localization in different Gossypium hirsutum genetic backgrounds. / Idris, Ali; Tuttle, John Richard; Robertson, Dominique Niki et al.
In: Physiological and Molecular Plant Pathology, Vol. 75, No. 1-2, 04.08.2010, p. 13-22.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Differential Cotton leaf crumple virus-VIGS-mediated gene silencing and viral genome localization in different Gossypium hirsutum genetic backgrounds

AU - Idris, Ali

AU - Tuttle, John Richard

AU - Robertson, Dominique Niki

AU - Haigler, Candace H.

AU - Brown, Judith K.

N1 - KAUST Repository Item: Exported on 2020-10-01

PY - 2010/8/4

Y1 - 2010/8/4

N2 - A Cotton leaf crumple virus (CLCrV)-based gene silencing vector containing a fragment of the Gossypium hirsutum Magnesium chelatase subunit I was used to establish endogenous gene silencing in cotton of varied genetic backgrounds. Biolistic inoculation resulted in systemic and persistent photo-bleaching of the leaves and bolls of the seven cultivars tested, however, the intensity of silencing was variable. CLCrV-VIGS-mediated expression of green fluorescent protein was used to monitor the in planta distribution of the vector, indicating successful phloem invasion in all cultivars tested. Acala SJ-1, one of the cotton cultivars, was identified as a particularly optimal candidate for CLCrV-VIGS-based cotton reverse-genetics. © 2010 Elsevier Ltd.

AB - A Cotton leaf crumple virus (CLCrV)-based gene silencing vector containing a fragment of the Gossypium hirsutum Magnesium chelatase subunit I was used to establish endogenous gene silencing in cotton of varied genetic backgrounds. Biolistic inoculation resulted in systemic and persistent photo-bleaching of the leaves and bolls of the seven cultivars tested, however, the intensity of silencing was variable. CLCrV-VIGS-mediated expression of green fluorescent protein was used to monitor the in planta distribution of the vector, indicating successful phloem invasion in all cultivars tested. Acala SJ-1, one of the cotton cultivars, was identified as a particularly optimal candidate for CLCrV-VIGS-based cotton reverse-genetics. © 2010 Elsevier Ltd.

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JO - Physiological and Molecular Plant Pathology

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Differential Cotton leaf crumple virus-VIGS-mediated gene silencing and viral genome localization in different Gossypium hirsutum genetic backgrounds

Abstract

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