Differential interference contrast x-ray microscopy with twin zone plates

Burkhard Kaulich; Thomas Wilhein; Enzo Di Fabrizio; Filippo Romanato; Matteo Altissimo; Stefano Cabrini; Barbara Fayard; Jean Susini

doi:10.1364/JOSAA.19.000797

Differential interference contrast x-ray microscopy with twin zone plates

Burkhard Kaulich^*, Thomas Wilhein, Enzo Di Fabrizio, Filippo Romanato, Matteo Altissimo, Stefano Cabrini, Barbara Fayard, Jean Susini

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

57 Scopus citations

Abstract

X-ray imaging in differential interference contrast (DIC) with submicrometer optical resolution was performed by using a twin zone plate (TZP) setup generating focal spots closely spaced within the TZP spatial resolution of 160 nm. Optical path differences introduced by the sample are recorded by a CCD camera in a standard full-field imaging and by an aperture photodiode in a standard scanning transmission x-ray microscope. Applying this x-ray DIC technique, we demonstrate for both the full-field imaging and scanning x-ray microscope methods a drastic increase in image contrast (approximately 20×) for a low-absorbing specimen, similar to the Nomarski DIC method for visible-light microscopy.

Original language	English (US)
Pages (from-to)	797-806
Number of pages	10
Journal	Journal of the Optical Society of America A: Optics and Image Science, and Vision
Volume	19
Issue number	4
DOIs	https://doi.org/10.1364/JOSAA.19.000797
State	Published - Apr 2002
Externally published	Yes

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Computer Vision and Pattern Recognition

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10.1364/JOSAA.19.000797

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title = "Differential interference contrast x-ray microscopy with twin zone plates",

abstract = "X-ray imaging in differential interference contrast (DIC) with submicrometer optical resolution was performed by using a twin zone plate (TZP) setup generating focal spots closely spaced within the TZP spatial resolution of 160 nm. Optical path differences introduced by the sample are recorded by a CCD camera in a standard full-field imaging and by an aperture photodiode in a standard scanning transmission x-ray microscope. Applying this x-ray DIC technique, we demonstrate for both the full-field imaging and scanning x-ray microscope methods a drastic increase in image contrast (approximately 20×) for a low-absorbing specimen, similar to the Nomarski DIC method for visible-light microscopy.",

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T1 - Differential interference contrast x-ray microscopy with twin zone plates

AU - Kaulich, Burkhard

AU - Wilhein, Thomas

AU - Di Fabrizio, Enzo

AU - Romanato, Filippo

AU - Altissimo, Matteo

AU - Cabrini, Stefano

AU - Fayard, Barbara

AU - Susini, Jean

PY - 2002/4

Y1 - 2002/4

N2 - X-ray imaging in differential interference contrast (DIC) with submicrometer optical resolution was performed by using a twin zone plate (TZP) setup generating focal spots closely spaced within the TZP spatial resolution of 160 nm. Optical path differences introduced by the sample are recorded by a CCD camera in a standard full-field imaging and by an aperture photodiode in a standard scanning transmission x-ray microscope. Applying this x-ray DIC technique, we demonstrate for both the full-field imaging and scanning x-ray microscope methods a drastic increase in image contrast (approximately 20×) for a low-absorbing specimen, similar to the Nomarski DIC method for visible-light microscopy.

AB - X-ray imaging in differential interference contrast (DIC) with submicrometer optical resolution was performed by using a twin zone plate (TZP) setup generating focal spots closely spaced within the TZP spatial resolution of 160 nm. Optical path differences introduced by the sample are recorded by a CCD camera in a standard full-field imaging and by an aperture photodiode in a standard scanning transmission x-ray microscope. Applying this x-ray DIC technique, we demonstrate for both the full-field imaging and scanning x-ray microscope methods a drastic increase in image contrast (approximately 20×) for a low-absorbing specimen, similar to the Nomarski DIC method for visible-light microscopy.

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Differential interference contrast x-ray microscopy with twin zone plates

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