TY - JOUR
T1 - Differential Marker Expression between Keratinocyte Stem Cells and Their Progeny Generated from a Single Colony.
AU - Ali, Dema
AU - Alhattab, Dana Majed
AU - Jafar, Hanan
AU - Alzubide, Malak
AU - Sharar, Nour
AU - Bdour, Salwa
AU - Awidi, Abdalla
N1 - KAUST Repository Item: Exported on 2021-10-21
Acknowledgements: This work was supported by the Scientific Research Fund/Ministry of Higher Education, Amman, Jordan, Grant # MPH/1/41/2015 and the Deanship of Scientific Research, The University of Jordan, Amman, Jordan.
PY - 2021/10/13
Y1 - 2021/10/13
N2 - The stemness in keratinocyte stem cells (KSCs) is determined by their gene expression patterns. KSCs are crucial in maintaining epidermal homeostasis and wound repair and are widely used candidates for therapeutic applications. Although several studies have reported their positive identifiers, unique biomarkers for KSCs remain elusive. Here, we aim to identify potential candidate stem cell markers. Human epidermal keratinocytes (HEKs) from neonatal foreskin tissues were isolated and cultured. Single-cell clonal analysis identified and characterized three types of cells: KSCs (holoclones), transient amplifying cells (TACs; meroclones), and differentiated cells (DSCs; paraclones). The clonogenic potential of KSCs demonstrated the highest proliferation potential of KSCs, followed by TACs and DSCs, respectively. Whole-transcriptome analysis using microarray technology unraveled the molecular signatures of these cells. These results were validated by quantitative real-time polymerase chain reaction and flow cytometry analysis. A total of 301 signature upregulated and 149 downregulated differentially expressed genes (DEGs) were identified in the KSCs, compared to TACs and DSCs. Furthermore, DEG analyses revealed new sets of genes related to cell proliferation, cell adhesion, surface makers, and regulatory factors. In conclusion, this study provides a useful source of information for the identification of potential SC-specific candidate markers.
AB - The stemness in keratinocyte stem cells (KSCs) is determined by their gene expression patterns. KSCs are crucial in maintaining epidermal homeostasis and wound repair and are widely used candidates for therapeutic applications. Although several studies have reported their positive identifiers, unique biomarkers for KSCs remain elusive. Here, we aim to identify potential candidate stem cell markers. Human epidermal keratinocytes (HEKs) from neonatal foreskin tissues were isolated and cultured. Single-cell clonal analysis identified and characterized three types of cells: KSCs (holoclones), transient amplifying cells (TACs; meroclones), and differentiated cells (DSCs; paraclones). The clonogenic potential of KSCs demonstrated the highest proliferation potential of KSCs, followed by TACs and DSCs, respectively. Whole-transcriptome analysis using microarray technology unraveled the molecular signatures of these cells. These results were validated by quantitative real-time polymerase chain reaction and flow cytometry analysis. A total of 301 signature upregulated and 149 downregulated differentially expressed genes (DEGs) were identified in the KSCs, compared to TACs and DSCs. Furthermore, DEG analyses revealed new sets of genes related to cell proliferation, cell adhesion, surface makers, and regulatory factors. In conclusion, this study provides a useful source of information for the identification of potential SC-specific candidate markers.
UR - http://hdl.handle.net/10754/672907
UR - https://www.mdpi.com/1422-0067/22/19/10810
UR - http://www.scopus.com/inward/record.url?scp=85116413161&partnerID=8YFLogxK
U2 - 10.3390/ijms221910810
DO - 10.3390/ijms221910810
M3 - Article
C2 - 34639148
SN - 1422-0067
VL - 22
SP - 10810
JO - International journal of molecular sciences
JF - International journal of molecular sciences
IS - 19
ER -