Digital holographic microscopy for the study of morphological changes in cells under simulated microgravity condition

C. Pache*, K. Westphal, J. Parent, A. Franco-Obregon, C. Depeursinge, M. Egli

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Previous investigations on mammalian cells have shown that real microgravity in space and/or simulated microgravity causes severe cellular modifications. Aim of the study was to use Digital Holographic Microscopy (DHM) to investigate morphological changes of cells under simulated microgravity generated by the Random Positioning Machine (RPM) in real-time. Preliminary studies were carried out on mouse myoblasts (C2C12). Besides showing cellular modification under simulated microgravity similar to the results obtained by using conventional methods, an increase of the phase height of the cell was measured which have not been reported yet. Our technique provides a new approach in the study of cellular adaptations to microgravity, providing real-time images of living cells.

Original languageEnglish (US)
Title of host publicationProceedings of the Symposium Life in Space for Life on Earth
PublisherEuropean Space Agency
ISBN (Print)9789292212278
StatePublished - 2008
Externally publishedYes
EventLife in Space for Life on Earth - Angers, France
Duration: Jun 22 2008Jul 27 2008

Publication series

NameEuropean Space Agency, (Special Publication) ESA SP
Volume663 SP
ISSN (Print)0379-6566

Other

OtherLife in Space for Life on Earth
Country/TerritoryFrance
CityAngers
Period06/22/0807/27/08

ASJC Scopus subject areas

  • Aerospace Engineering
  • Space and Planetary Science

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