Abstract
We describe the fabrication of an amperometric biosensor based on cytochrome c (Cyt c) immobilized graphene oxide-multiwalled carbon nanotube (GO-MWCNT) composite on a nano Au modified glassy carbon electrode for trace level detection of H2O2. Morphology and surface characterization of the nanocomposite reveal the successful formation of a highly conducting MWCNT network on the GO surface. Electrochemical impedance studies indicate a lower charge transfer resistance compared to the bare electrode. Cyclic voltammetry studies clearly demonstrate an enhanced direct electrochemistry of Cyt c with a high electron transfer rate constant (k s) value of 3.4 s-1. An amperometric H2O 2 biosensor has been fabricated with an excellent current sensitivity of 0.533 μA pM-1 cm-2 and a very low detection limit of 27.7 pM. The fabricated sensor shows exceptional selectivity to H 2O2 in the presence of a high concentration of some likely interferents. Moreover, the sensor exhibits high stability with appreciable repeatability and reproducibility. © 2014 the Partner Organisations.
Original language | English (US) |
---|---|
Pages (from-to) | 28229-28237 |
Number of pages | 9 |
Journal | RSC ADVANCES |
Volume | 4 |
Issue number | 54 |
DOIs | |
State | Published - Jan 1 2014 |
Externally published | Yes |
ASJC Scopus subject areas
- General Chemical Engineering
- General Chemistry