Disruption of the cytochrome CYP711A5 gene reveals MAX1 redundancy in rice strigolactone biosynthesis

Jian You Wang; Guan Ting Erica Chen; Justine Braguy; Muhammad Jamil; Lamis Berqdar; Salim Al-Babili

doi:10.1016/j.jplph.2023.154057

Disruption of the cytochrome CYP711A5 gene reveals MAX1 redundancy in rice strigolactone biosynthesis

Jian You Wang, Guan Ting Erica Chen, Justine Braguy, Muhammad Jamil, Lamis Berqdar, Salim Al-Babili^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Strigolactones (SLs) inhibit shoot branching/tillering and are secreted by plant roots as a signal to attract symbiotic mycorrhizal fungi in the rhizosphere, particularly under phosphate starvation. However, SLs are also hijacked by root parasitic weeds as inducer for the germination of their seeds. There are around 35 natural SLs divided, based on their structures, into canonical and non-canonical SLs. Cytochrome P450 enzymes of the 711 clade, such as MORE AXILLARY GROWTH1 (MAX1) in Arabidopsis, are a major driver of SL structural diversity. Monocots, such as rice, contain several MAX1 homologs that participate in SL biosynthesis. To investigate the function of OsMAX1-1900 in planta, we generated CRISPR/Cas9 mutants disrupted in the corresponding gene. Characterizing of the generated mutants at metabolite and phenotype level suggests that OsMAX1-1900 loss-of-function does neither affect the SL pattern nor rice architecture, indicating functional redundancy among rice MAX1 homologs.

Original language	English (US)
Article number	154057
Journal	Journal of Plant Physiology
Volume	287
DOIs	https://doi.org/10.1016/j.jplph.2023.154057
State	Published - Aug 2023

Keywords

Cytochrome P450
MORE AXILLARY GROWTH1
Plant architecture
Striga
Strigolactones

ASJC Scopus subject areas

Physiology
Agronomy and Crop Science
Plant Science

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10.1016/j.jplph.2023.154057

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title = "Disruption of the cytochrome CYP711A5 gene reveals MAX1 redundancy in rice strigolactone biosynthesis",

abstract = "Strigolactones (SLs) inhibit shoot branching/tillering and are secreted by plant roots as a signal to attract symbiotic mycorrhizal fungi in the rhizosphere, particularly under phosphate starvation. However, SLs are also hijacked by root parasitic weeds as inducer for the germination of their seeds. There are around 35 natural SLs divided, based on their structures, into canonical and non-canonical SLs. Cytochrome P450 enzymes of the 711 clade, such as MORE AXILLARY GROWTH1 (MAX1) in Arabidopsis, are a major driver of SL structural diversity. Monocots, such as rice, contain several MAX1 homologs that participate in SL biosynthesis. To investigate the function of OsMAX1-1900 in planta, we generated CRISPR/Cas9 mutants disrupted in the corresponding gene. Characterizing of the generated mutants at metabolite and phenotype level suggests that OsMAX1-1900 loss-of-function does neither affect the SL pattern nor rice architecture, indicating functional redundancy among rice MAX1 homologs.",

keywords = "Cytochrome P450, MORE AXILLARY GROWTH1, Plant architecture, Striga, Strigolactones",

author = "Wang, {Jian You} and Chen, {Guan Ting Erica} and Justine Braguy and Muhammad Jamil and Lamis Berqdar and Salim Al-Babili",

note = "Funding Information: We sincerely thank the members of KAUST Analytical Core Lab and the Bioactives lab for their support. We thank Dr. Abdel Gabbar Babiker for providing Striga hermonthica seeds. This work was supported by baseline funding given to S. A-B from King Abdullah University of Science and Technology ( KAUST ) and the Bill and Melinda Gates Foundation (grant number OPP1136424 ). Publisher Copyright: {\textcopyright} 2023 Elsevier GmbH",

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language = "English (US)",

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AU - Wang, Jian You

AU - Chen, Guan Ting Erica

AU - Braguy, Justine

AU - Jamil, Muhammad

AU - Berqdar, Lamis

AU - Al-Babili, Salim

N1 - Funding Information: We sincerely thank the members of KAUST Analytical Core Lab and the Bioactives lab for their support. We thank Dr. Abdel Gabbar Babiker for providing Striga hermonthica seeds. This work was supported by baseline funding given to S. A-B from King Abdullah University of Science and Technology ( KAUST ) and the Bill and Melinda Gates Foundation (grant number OPP1136424 ). Publisher Copyright: © 2023 Elsevier GmbH

PY - 2023/8

Y1 - 2023/8

N2 - Strigolactones (SLs) inhibit shoot branching/tillering and are secreted by plant roots as a signal to attract symbiotic mycorrhizal fungi in the rhizosphere, particularly under phosphate starvation. However, SLs are also hijacked by root parasitic weeds as inducer for the germination of their seeds. There are around 35 natural SLs divided, based on their structures, into canonical and non-canonical SLs. Cytochrome P450 enzymes of the 711 clade, such as MORE AXILLARY GROWTH1 (MAX1) in Arabidopsis, are a major driver of SL structural diversity. Monocots, such as rice, contain several MAX1 homologs that participate in SL biosynthesis. To investigate the function of OsMAX1-1900 in planta, we generated CRISPR/Cas9 mutants disrupted in the corresponding gene. Characterizing of the generated mutants at metabolite and phenotype level suggests that OsMAX1-1900 loss-of-function does neither affect the SL pattern nor rice architecture, indicating functional redundancy among rice MAX1 homologs.

AB - Strigolactones (SLs) inhibit shoot branching/tillering and are secreted by plant roots as a signal to attract symbiotic mycorrhizal fungi in the rhizosphere, particularly under phosphate starvation. However, SLs are also hijacked by root parasitic weeds as inducer for the germination of their seeds. There are around 35 natural SLs divided, based on their structures, into canonical and non-canonical SLs. Cytochrome P450 enzymes of the 711 clade, such as MORE AXILLARY GROWTH1 (MAX1) in Arabidopsis, are a major driver of SL structural diversity. Monocots, such as rice, contain several MAX1 homologs that participate in SL biosynthesis. To investigate the function of OsMAX1-1900 in planta, we generated CRISPR/Cas9 mutants disrupted in the corresponding gene. Characterizing of the generated mutants at metabolite and phenotype level suggests that OsMAX1-1900 loss-of-function does neither affect the SL pattern nor rice architecture, indicating functional redundancy among rice MAX1 homologs.

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Disruption of the cytochrome CYP711A5 gene reveals MAX1 redundancy in rice strigolactone biosynthesis

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Keywords

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