TY - JOUR
T1 - Dissociation coefficients of protein adsorption to nanoparticles as quantitative metrics for description of the protein corona: A comparison of experimental techniques and methodological relevance
AU - Hühn, Jonas
AU - Fedeli, Chiara
AU - Zhang, Qian
AU - Masood, Atif
AU - del Pino, Pablo
AU - Khashab, Niveen M.
AU - Papini, Emanuele
AU - Parak, Wolfgang J.
N1 - KAUST Repository Item: Exported on 2020-10-01
PY - 2016/1/1
Y1 - 2016/1/1
N2 - Protein adsorption to nanoparticles is described as a chemical reaction in which proteins attach to binding sites on the nanoparticle surface. This process can be described with a dissociation coefficient, which tells how many proteins are adsorbed per nanoparticle in dependence of the protein concentration. Different techniques to experimentally determine dissociation coefficients of protein adsorption to nanoparticles are reviewed. Results of more than 130 experiments in which dissociation coefficients have been determined are compared. Data show that different methods, nanoparticle systems, and proteins can lead to significantly different dissociation coefficients. However, we observed a clear tendency of smaller dissociation coefficients upon less negative towards more positive zeta potentials of the nanoparticles. The zeta potential thus is a key parameter influencing protein adsorption to the surface of nanoparticles. Our analysis highlights the importance of the characterization of the parameters governing protein-nanoparticle interaction for quantitative evaluation and objective literature comparison.
AB - Protein adsorption to nanoparticles is described as a chemical reaction in which proteins attach to binding sites on the nanoparticle surface. This process can be described with a dissociation coefficient, which tells how many proteins are adsorbed per nanoparticle in dependence of the protein concentration. Different techniques to experimentally determine dissociation coefficients of protein adsorption to nanoparticles are reviewed. Results of more than 130 experiments in which dissociation coefficients have been determined are compared. Data show that different methods, nanoparticle systems, and proteins can lead to significantly different dissociation coefficients. However, we observed a clear tendency of smaller dissociation coefficients upon less negative towards more positive zeta potentials of the nanoparticles. The zeta potential thus is a key parameter influencing protein adsorption to the surface of nanoparticles. Our analysis highlights the importance of the characterization of the parameters governing protein-nanoparticle interaction for quantitative evaluation and objective literature comparison.
UR - http://hdl.handle.net/10754/592742
UR - http://linkinghub.elsevier.com/retrieve/pii/S135727251530087X
UR - http://www.scopus.com/inward/record.url?scp=84956609671&partnerID=8YFLogxK
U2 - 10.1016/j.biocel.2015.12.015
DO - 10.1016/j.biocel.2015.12.015
M3 - Article
C2 - 26748245
SN - 1357-2725
VL - 75
SP - 148
EP - 161
JO - The International Journal of Biochemistry & Cell Biology
JF - The International Journal of Biochemistry & Cell Biology
ER -