Division of Labor

Muse Oke, Manal Zaher, Samir Hamdan

Research output: Chapter in Book/Report/Conference proceedingChapter


The first assignment of DNA polymerases at the eukaryotic replication fork was possible after the in vitro reconstitution of the simian virus 40 (SV40) replication system. In this system, DNA polymerase α (Pol α) provides both leading and lagging strands with RNA-DNA primers that are extended by DNA polymerase δ (Pol δ). Extrapolating the architecture of the replication fork from the SV40 model system to an actual eukaryotic cell has been challenged by the discovery of a third DNA polymerase in Saccharomyces cerevisiae, DNA polymerase ε (Pol ε). A division of labor has been proposed for the eukaryotic replication fork whereby Pol ε replicates the leading strand and Pol δ replicates the lagging strand. However, an alternative model of unequal division of labor in which Pol δ can still participate in leading-strand synthesis is plausible.
Original languageEnglish (US)
Title of host publicationMolecular Life Sciences
PublisherSpringer Nature
Number of pages5
ISBN (Print)9781461464365
StatePublished - Jul 14 2014


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