Do-it-yourself guide: How to use the modern single-molecule toolkit

Nils G. Walter; Cheng Yen Huang; Anthony J. Manzo; Mohamed A. Sobhy

doi:10.1038/nmeth.1215

Do-it-yourself guide: How to use the modern single-molecule toolkit

Nils G. Walter^*, Cheng Yen Huang, Anthony J. Manzo, Mohamed A. Sobhy

^*Corresponding author for this work

Research output: Contribution to journal › Review article › peer-review

277 Scopus citations

Abstract

Single-molecule microscopy has evolved into the ultimate-sensitivity toolkit to study systems from small molecules to living cells, with the prospect of revolutionizing the modern biosciences. Here we survey the current state of the art in single-molecule tools including fluorescence spectroscopy, tethered particle microscopy, optical and magnetic tweezers, and atomic force microscopy. We also provide guidelines for choosing the right approach from the available single-molecule toolkit for applications as diverse as structural biology, enzymology, nanotechnology and systems biology.

Original language	English (US)
Pages (from-to)	475-489
Number of pages	15
Journal	Nature Methods
Volume	5
Issue number	6
DOIs	https://doi.org/10.1038/nmeth.1215
State	Published - Jun 2008
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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Do-it-yourself guide: How to use the modern single-molecule toolkit

Abstract

ASJC Scopus subject areas

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