TY - JOUR
T1 - Down-regulated expression of OPCML predicts an unfavorable prognosis and promotes disease progression in human gastric cancer
AU - Xing, Xiangbin
AU - Cai, Weibin
AU - Ma, Sanmei
AU - Wang, Yongfei
AU - Shi, Huijuan
AU - Li, Ming
AU - Jiao, Jinxia
AU - Yang, Yang
AU - Liu, Longshan
AU - Zhang, Xiangliang
AU - Chen, Minhu
N1 - Generated from Scopus record by KAUST IRTS on 2023-09-20
PY - 2017/4/14
Y1 - 2017/4/14
N2 - Background: OPCML belongs to the IgLON family of Ig domain-containing GPI-anchored cell adhesion molecules and was recently found to be involved in carcinogenesis, while its role in gastric cancer remains unclear. Methods: We assessed expression and biological behavior of OPCML in gastric cancer. Results: OPCML expression was markedly reduced in tumor tissues and cancer cell lines. Decreased OPCML expression had a significant association with unfavorable tumor stage (p = 0.007) and grading (p < 0.001). Furthermore, the results revealed that OPCML was an independent prognostic factor for overall survival in gastric cancer (p = 0.002). In addition, ectopic expression of OPCML in cancer cells significantly inhibited cell viability (p < 0.01) and colony formation (p < 0.001), arrest cell cycle in G0/G1 phase and induced apoptosis, and suppressed tumor formation in nude mice. The alterations of phosphorylation status of AKT and its substrate GSK3β, up-regulation of pro-apoptotic regulators including caspase-3, caspase-9 and PARP, and up-regulation of cell cycle regulator p27, were implicated in the biological activity of OPCML in cancer cells. Conclusion: Down-regulated OPCML expression might serve as an independent predictor for unfavorable prognosis of patients, and the biological behavior supports its role as a tumor suppressor in gastric cancer.
AB - Background: OPCML belongs to the IgLON family of Ig domain-containing GPI-anchored cell adhesion molecules and was recently found to be involved in carcinogenesis, while its role in gastric cancer remains unclear. Methods: We assessed expression and biological behavior of OPCML in gastric cancer. Results: OPCML expression was markedly reduced in tumor tissues and cancer cell lines. Decreased OPCML expression had a significant association with unfavorable tumor stage (p = 0.007) and grading (p < 0.001). Furthermore, the results revealed that OPCML was an independent prognostic factor for overall survival in gastric cancer (p = 0.002). In addition, ectopic expression of OPCML in cancer cells significantly inhibited cell viability (p < 0.01) and colony formation (p < 0.001), arrest cell cycle in G0/G1 phase and induced apoptosis, and suppressed tumor formation in nude mice. The alterations of phosphorylation status of AKT and its substrate GSK3β, up-regulation of pro-apoptotic regulators including caspase-3, caspase-9 and PARP, and up-regulation of cell cycle regulator p27, were implicated in the biological activity of OPCML in cancer cells. Conclusion: Down-regulated OPCML expression might serve as an independent predictor for unfavorable prognosis of patients, and the biological behavior supports its role as a tumor suppressor in gastric cancer.
UR - http://bmccancer.biomedcentral.com/articles/10.1186/s12885-017-3203-y
UR - http://www.scopus.com/inward/record.url?scp=85018466348&partnerID=8YFLogxK
U2 - 10.1186/s12885-017-3203-y
DO - 10.1186/s12885-017-3203-y
M3 - Article
SN - 1471-2407
VL - 17
JO - BMC Cancer
JF - BMC Cancer
IS - 1
ER -
