Dynamics of isoelectric precipitation of casein using sulfuric acid

G. W. Hofland*, M. R. Berkhoff, G. J. Witkamp, L. A.M. Van der Wielen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


The acid precipitation of casein from skim milk is an interesting and complex operation, as it involves several mechanistic steps occurring simultaneously. Research has focused so far on the influence of static variables such as temperature and final pH on the curd properties. Here the dynamics of the process was investigated. From a characteristic times analysis it was concluded that the most important mechanistic steps in the acid precipitation of casein were acid mixing, aggregation/breakup, and transport in/out of the precipitate particles. Experiments in a fed batch setup using sulfuric acid as the precipitant showed how the precipitation influenced the pH profile and demonstrated the large influence of process variables, such as the acid addition rate, the mixing intensity, and aging time on particle-size distributions and the release of minerals from the casein.

Original languageEnglish (US)
Pages (from-to)2211-2223
Number of pages13
JournalAIChE Journal
Issue number8
StatePublished - Aug 1 2003
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Environmental Engineering
  • Chemical Engineering(all)


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