TY - JOUR
T1 - Effect of polybrominated diphenyl ether (PBDE) treatment on the composition and function of the bacterial community in the sponge Haliclona cymaeformis.
AU - Tian, Ren-Mao
AU - Lee, On On
AU - Wang, Yong
AU - Cai, Lin
AU - Bougouffa, Salim
AU - Chiu, Jill Man Ying
AU - Wu, Rudolf Shiu Sun
AU - Qian, Pei-Yuan
N1 - KAUST Repository Item: Exported on 2020-10-01
Acknowledged KAUST grant number(s): SA-C0040, UK-C0016
Acknowledgements: This research is supported by the National Basic Research Program of China (973 Program, No: 2012CB417304), State Key Laboratory of Marine Pollution (SKLMP) Seed Collaborative Research fund (CITYU12SC01), the Area of Excellence (AoE) fund (AoE04/04-II) and an award (SA-C0040/UK-C0016) from the King Abdullah University of Science and Technology granted to Pei-Yuan Qian.
This publication acknowledges KAUST support, but has no KAUST affiliated authors.
PY - 2015/1/14
Y1 - 2015/1/14
N2 - Marine sponges play important roles in benthic environments and are sensitive to environmental stresses. Polybrominated diphenyl ethers (PBDEs) have been widely used as flame retardants since the 1970s and are cytotoxic and genotoxic to organisms. In the present study, we studied the short-period effect of PBDE-47 (2,2',4,4'-tetrabromodiphenyl ether) treatment on the community structure and functional gene composition of the bacterial community inhabiting the marine sponge Haliclona cymaeformis. Our results showed that the bacterial community shifted from an autotrophic bacteria-dominated community to a heterotrophic bacteria-dominated community in response to PBDE-47 in a time- and concentration-dependent manner. A potentially symbiotic sulfur-oxidizing bacterium (SOB) was dominant (>80% in abundance) in the untreated sponge. However, exposure to a high concentration (1 μg/L) of PBDE-47 caused a substantial decrease in the potential symbiont and an enrichment of heterotrophic bacteria like Clostridium. A metagenomic analysis showed a selective effect of the high concentration treatment on the functional gene composition of the enriched heterotrophic bacteria, revealing an enrichment for the functions responsible for DNA repair, multidrug efflux pumping, and bacterial chemotaxis and motility. This study demonstrated that PBDE-47 induced a shift in the composition of the community and functional genes in the sponge-associated bacterial community, revealing the selective effect of PBDE-47 treatment on the functions of the bacterial community in the microenvironment of the sponge.
AB - Marine sponges play important roles in benthic environments and are sensitive to environmental stresses. Polybrominated diphenyl ethers (PBDEs) have been widely used as flame retardants since the 1970s and are cytotoxic and genotoxic to organisms. In the present study, we studied the short-period effect of PBDE-47 (2,2',4,4'-tetrabromodiphenyl ether) treatment on the community structure and functional gene composition of the bacterial community inhabiting the marine sponge Haliclona cymaeformis. Our results showed that the bacterial community shifted from an autotrophic bacteria-dominated community to a heterotrophic bacteria-dominated community in response to PBDE-47 in a time- and concentration-dependent manner. A potentially symbiotic sulfur-oxidizing bacterium (SOB) was dominant (>80% in abundance) in the untreated sponge. However, exposure to a high concentration (1 μg/L) of PBDE-47 caused a substantial decrease in the potential symbiont and an enrichment of heterotrophic bacteria like Clostridium. A metagenomic analysis showed a selective effect of the high concentration treatment on the functional gene composition of the enriched heterotrophic bacteria, revealing an enrichment for the functions responsible for DNA repair, multidrug efflux pumping, and bacterial chemotaxis and motility. This study demonstrated that PBDE-47 induced a shift in the composition of the community and functional genes in the sponge-associated bacterial community, revealing the selective effect of PBDE-47 treatment on the functions of the bacterial community in the microenvironment of the sponge.
UR - http://hdl.handle.net/10754/596782
UR - http://journal.frontiersin.org/article/10.3389/fmicb.2014.00799/abstract
UR - http://www.scopus.com/inward/record.url?scp=84920681943&partnerID=8YFLogxK
U2 - 10.3389/fmicb.2014.00799
DO - 10.3389/fmicb.2014.00799
M3 - Article
C2 - 25642227
SN - 1664-302X
VL - 5
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
IS - DEC
ER -