Effects of voltage-gated calcium channel subunit genes on calcium influx in cultured C. elegans mechanosensory neurons

Christian Frøkjær-Jensen, Katie S. Kindt, Rex A. Kerr, Hiroshi Suzuki, Katya Melnik-Martinez, Beate Gerstbreih, Monica Driscol, William R. Schafer*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

44 Scopus citations


Voltage-gated calcium channels (VGCCs) serve as a critical link between electrical signaling and diverse cellular processes in neurons. We have exploited recent advances in genetically encoded calcium sensors and in culture techniques to investigate how the VGCC α1 subunit EGL-19 and α2/δ subunit UNC-36 affect the functional properties of C. elegans mechanosensory neurons. Using the protein-based optical indicator cameleon, we recorded calcium transients from cultured mechanosensory neurons in response to transient depolarization. We observed that in these cultured cells, calcium transients induced by extracellular potassium were significantly reduced by a reduction-of-function mutation in egl-19 and significantly reduced by L-type calcium channel inhibitors; thus, a main source of touch neuron calcium transients appeared to be influx of extracellular calcium through L-type channels. Transients did not depend directly on intracellular calcium stores, although a store-independent 2-APB and gadolinium-sensitive calcium flux was detected. The transients were also significantly reduced by mutations in unc-36, which encodes the main neuronal α2/δ subunit in C. elegans. Interestingly, while egl-19 mutations resulted in similar reductions in calcium influx at all stimulus strengths, unc-36 mutations preferentially affected responses to smaller depolarizations. These experiments suggest a central role for EGL-19 and UNC-36 in excitability and functional activity of the mechanosensory neurons.

Original languageEnglish (US)
Pages (from-to)1125-1139
Number of pages15
JournalJournal of Neurobiology
Issue number10
StatePublished - Sep 2006
Externally publishedYes


  • C. elegans
  • Calcium channel
  • Genetics
  • Mechanosensation
  • Neuroimaging

ASJC Scopus subject areas

  • General Neuroscience
  • Cellular and Molecular Neuroscience


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