Efficient separation of small molecules using a large surface area hypercrosslinked monolithic polymer capillary column

Jiri Urban; Frantisek Svec; Jean M.J. Fréchet

doi:10.1021/ac100008n

Efficient separation of small molecules using a large surface area hypercrosslinked monolithic polymer capillary column

Jiri Urban, Frantisek Svec, Jean M.J. Fréchet

Research output: Contribution to journal › Article › peer-review

143 Scopus citations

Abstract

Monolithic poly(styrene-co-vinylbenzyl chloride-co-divinylbenzene) precursor capillary columns have been prepared and then hypercrosslinked to afford a monolith containing an array of small pores. This monolithic column exhibited a surface area of 663 m²/g or more than 1 order of magnitude larger than measured for the precursor column. Because it contains mesopores, this monolithic column affords good separation of uracil and alkylbenzenes in isocratic mobile phase mode and also proved useful for separations in size exclusion mode. A column efficiency as high as 73000 plates/m was determined for uracil. In contrast, the presence of mesopores in this hypercrosslinked monolithic column had a detrimental effect on the separation of proteins.

Original language	English (US)
Pages (from-to)	1621-1623
Number of pages	3
Journal	ANALYTICAL CHEMISTRY
Volume	82
Issue number	5
DOIs	https://doi.org/10.1021/ac100008n
State	Published - Mar 1 2010
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry

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title = "Efficient separation of small molecules using a large surface area hypercrosslinked monolithic polymer capillary column",

abstract = "Monolithic poly(styrene-co-vinylbenzyl chloride-co-divinylbenzene) precursor capillary columns have been prepared and then hypercrosslinked to afford a monolith containing an array of small pores. This monolithic column exhibited a surface area of 663 m2/g or more than 1 order of magnitude larger than measured for the precursor column. Because it contains mesopores, this monolithic column affords good separation of uracil and alkylbenzenes in isocratic mobile phase mode and also proved useful for separations in size exclusion mode. A column efficiency as high as 73000 plates/m was determined for uracil. In contrast, the presence of mesopores in this hypercrosslinked monolithic column had a detrimental effect on the separation of proteins.",

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AU - Svec, Frantisek

AU - Fréchet, Jean M.J.

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N2 - Monolithic poly(styrene-co-vinylbenzyl chloride-co-divinylbenzene) precursor capillary columns have been prepared and then hypercrosslinked to afford a monolith containing an array of small pores. This monolithic column exhibited a surface area of 663 m2/g or more than 1 order of magnitude larger than measured for the precursor column. Because it contains mesopores, this monolithic column affords good separation of uracil and alkylbenzenes in isocratic mobile phase mode and also proved useful for separations in size exclusion mode. A column efficiency as high as 73000 plates/m was determined for uracil. In contrast, the presence of mesopores in this hypercrosslinked monolithic column had a detrimental effect on the separation of proteins.

AB - Monolithic poly(styrene-co-vinylbenzyl chloride-co-divinylbenzene) precursor capillary columns have been prepared and then hypercrosslinked to afford a monolith containing an array of small pores. This monolithic column exhibited a surface area of 663 m2/g or more than 1 order of magnitude larger than measured for the precursor column. Because it contains mesopores, this monolithic column affords good separation of uracil and alkylbenzenes in isocratic mobile phase mode and also proved useful for separations in size exclusion mode. A column efficiency as high as 73000 plates/m was determined for uracil. In contrast, the presence of mesopores in this hypercrosslinked monolithic column had a detrimental effect on the separation of proteins.

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Efficient separation of small molecules using a large surface area hypercrosslinked monolithic polymer capillary column

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