TY - JOUR
T1 - ELABELA: A hormone essential for heart development signals via the apelin receptor
AU - Chng, Serene C.
AU - Ho, Lena
AU - Tian, Jing
AU - Reversade, Bruno
N1 - Generated from Scopus record by KAUST IRTS on 2023-02-15
PY - 2013/12/23
Y1 - 2013/12/23
N2 - We report here the discovery and characterization ofa gene, ELABELA (ELA), encoding a conserved hormone of 32 amino acids. Present in human embryonic stem cells, ELA is expressed at the onset of zebrafish zygotic transcription and is ubiquitous in the naive ectodermal cells of the embryo. Using zinc-finger-nuclease-mediated gene inactivation in zebrafish, we created an allelic series of ela mutants. ela null embryos have impaired endoderm differentiation potential marked by reduced gata5 and sox17 expression. Loss of Ela causes embryos to develop with a rudimentary heart or no heart at all, surprisingly phenocopying the loss of the apelin receptor (aplnr), which we show serves as Ela's cognate G protein-coupled receptor. Our results reveal the existence of apeptide hormone, ELA, which, together with APLNR, forms an essential signaling axis for early cardiovascular development. © 2013 Elsevier Inc.
AB - We report here the discovery and characterization ofa gene, ELABELA (ELA), encoding a conserved hormone of 32 amino acids. Present in human embryonic stem cells, ELA is expressed at the onset of zebrafish zygotic transcription and is ubiquitous in the naive ectodermal cells of the embryo. Using zinc-finger-nuclease-mediated gene inactivation in zebrafish, we created an allelic series of ela mutants. ela null embryos have impaired endoderm differentiation potential marked by reduced gata5 and sox17 expression. Loss of Ela causes embryos to develop with a rudimentary heart or no heart at all, surprisingly phenocopying the loss of the apelin receptor (aplnr), which we show serves as Ela's cognate G protein-coupled receptor. Our results reveal the existence of apeptide hormone, ELA, which, together with APLNR, forms an essential signaling axis for early cardiovascular development. © 2013 Elsevier Inc.
UR - https://linkinghub.elsevier.com/retrieve/pii/S1534580713006655
UR - http://www.scopus.com/inward/record.url?scp=84890856728&partnerID=8YFLogxK
U2 - 10.1016/j.devcel.2013.11.002
DO - 10.1016/j.devcel.2013.11.002
M3 - Article
SN - 1534-5807
VL - 27
SP - 672
EP - 680
JO - Developmental Cell
JF - Developmental Cell
IS - 6
ER -