Enhancing plasma peptide MALDI-TOF-MS profiling by mesoporous silica assisted crystallization

Rosa Terracciano; Francesca Casadonte; Luigi Pasqua; Patrizio Candeloro; Enzo Di Fabrizio; Andrea Urbani; Rocco Savino

doi:10.1016/j.talanta.2009.03.060

Enhancing plasma peptide MALDI-TOF-MS profiling by mesoporous silica assisted crystallization

Rosa Terracciano^*, Francesca Casadonte, Luigi Pasqua, Patrizio Candeloro, Enzo Di Fabrizio, Andrea Urbani, Rocco Savino

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Promising profiling techniques based on new material/solid phase extraction for capturing "molecular signatures" from body fluids are being coupled to MALDI-TOF-MS. Sample preparation significantly influences spectrum quality in this ionization method. Mesoporous silica beads (MSB), by the means of nano-sized porous channels with high surface area, enable harvesting of peptides from plasma and serum excluding large size proteins. We have investigated the morphology of a sample slurry, developed as a new tool for plasma peptides enrichment based on mesoporous materials. Our study highlights a correlation between crystals morphology and enhanced performances in MALDI-TOF-MS analysis. This is the first report which correlates the increase in signal intensity with crystal formation in samples preparations which make use of various kinds of slurries for the analysis of samples clinically relevant like human plasma.

Original language	English (US)
Pages (from-to)	1532-1538
Number of pages	7
Journal	Talanta
Volume	80
Issue number	4
DOIs	https://doi.org/10.1016/j.talanta.2009.03.060
State	Published - Feb 15 2010
Externally published	Yes

Keywords

Biomarkers discovery
Clinical proteomics
Human plasma
MALDI-TOF-MS
Mesoporous materials

ASJC Scopus subject areas

Analytical Chemistry

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10.1016/j.talanta.2009.03.060

Cite this

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title = "Enhancing plasma peptide MALDI-TOF-MS profiling by mesoporous silica assisted crystallization",

abstract = "Promising profiling techniques based on new material/solid phase extraction for capturing {"}molecular signatures{"} from body fluids are being coupled to MALDI-TOF-MS. Sample preparation significantly influences spectrum quality in this ionization method. Mesoporous silica beads (MSB), by the means of nano-sized porous channels with high surface area, enable harvesting of peptides from plasma and serum excluding large size proteins. We have investigated the morphology of a sample slurry, developed as a new tool for plasma peptides enrichment based on mesoporous materials. Our study highlights a correlation between crystals morphology and enhanced performances in MALDI-TOF-MS analysis. This is the first report which correlates the increase in signal intensity with crystal formation in samples preparations which make use of various kinds of slurries for the analysis of samples clinically relevant like human plasma.",

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doi = "10.1016/j.talanta.2009.03.060",

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AU - Terracciano, Rosa

AU - Casadonte, Francesca

AU - Pasqua, Luigi

AU - Candeloro, Patrizio

AU - Di Fabrizio, Enzo

AU - Urbani, Andrea

AU - Savino, Rocco

PY - 2010/2/15

Y1 - 2010/2/15

N2 - Promising profiling techniques based on new material/solid phase extraction for capturing "molecular signatures" from body fluids are being coupled to MALDI-TOF-MS. Sample preparation significantly influences spectrum quality in this ionization method. Mesoporous silica beads (MSB), by the means of nano-sized porous channels with high surface area, enable harvesting of peptides from plasma and serum excluding large size proteins. We have investigated the morphology of a sample slurry, developed as a new tool for plasma peptides enrichment based on mesoporous materials. Our study highlights a correlation between crystals morphology and enhanced performances in MALDI-TOF-MS analysis. This is the first report which correlates the increase in signal intensity with crystal formation in samples preparations which make use of various kinds of slurries for the analysis of samples clinically relevant like human plasma.

AB - Promising profiling techniques based on new material/solid phase extraction for capturing "molecular signatures" from body fluids are being coupled to MALDI-TOF-MS. Sample preparation significantly influences spectrum quality in this ionization method. Mesoporous silica beads (MSB), by the means of nano-sized porous channels with high surface area, enable harvesting of peptides from plasma and serum excluding large size proteins. We have investigated the morphology of a sample slurry, developed as a new tool for plasma peptides enrichment based on mesoporous materials. Our study highlights a correlation between crystals morphology and enhanced performances in MALDI-TOF-MS analysis. This is the first report which correlates the increase in signal intensity with crystal formation in samples preparations which make use of various kinds of slurries for the analysis of samples clinically relevant like human plasma.

KW - Biomarkers discovery

KW - Clinical proteomics

KW - Human plasma

KW - MALDI-TOF-MS

KW - Mesoporous materials

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Enhancing plasma peptide MALDI-TOF-MS profiling by mesoporous silica assisted crystallization

Abstract

Keywords

ASJC Scopus subject areas

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