Enzyme microarrays: On-chip determination of inhibition constants based on affinity-label detection of enzymatic activity

Jörg Eppinger; Daniel P. Funeriu; Masato Miyake; Lucile Denizot; Jun Miyake

doi:10.1002/anie.200353623

Enzyme microarrays: On-chip determination of inhibition constants based on affinity-label detection of enzymatic activity

Jörg Eppinger^*, Daniel P. Funeriu, Masato Miyake, Lucile Denizot, Jun Miyake

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

High-throughput screening of libraries of potential inhibitors is possible by using an enzyme microarray method that combines low sample consumption with accuracy and speed. Information about enzyme inhibition can be obtained "on-chip" by the addition of a fluorescently tagged affinity label to a microarray of functional enzymes (see picture).

Original language	English (US)
Pages (from-to)	3806-3810
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	43
Issue number	29
DOIs	https://doi.org/10.1002/anie.200353623
State	Published - Jul 19 2004
Externally published	Yes

Keywords

Enzymes
Fluorescent probes
High-throughput screening
Inhibitors
Microarrays
On-chip kinetics

ASJC Scopus subject areas

Catalysis
General Chemistry

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10.1002/anie.200353623

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abstract = "High-throughput screening of libraries of potential inhibitors is possible by using an enzyme microarray method that combines low sample consumption with accuracy and speed. Information about enzyme inhibition can be obtained {"}on-chip{"} by the addition of a fluorescently tagged affinity label to a microarray of functional enzymes (see picture).",

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AU - Funeriu, Daniel P.

AU - Miyake, Masato

AU - Denizot, Lucile

AU - Miyake, Jun

PY - 2004/7/19

Y1 - 2004/7/19

N2 - High-throughput screening of libraries of potential inhibitors is possible by using an enzyme microarray method that combines low sample consumption with accuracy and speed. Information about enzyme inhibition can be obtained "on-chip" by the addition of a fluorescently tagged affinity label to a microarray of functional enzymes (see picture).

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KW - Enzymes

KW - Fluorescent probes

KW - High-throughput screening

KW - Inhibitors

KW - Microarrays

KW - On-chip kinetics

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AN - SCOPUS:4444290143

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Enzyme microarrays: On-chip determination of inhibition constants based on affinity-label detection of enzymatic activity

Abstract

Keywords

ASJC Scopus subject areas

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