TY - JOUR
T1 - Estrogen induces shift in abundances of specific groups of the coral microbiome
T2 - Scientific Reports
AU - Vilela, Caren L. S.
AU - Villela, Helena D. M.
AU - Duarte, Gustavo A. S.
AU - Santoro, Erika P.
AU - Rachid, Caio T. C. C.
AU - Peixoto, Raquel S.
N1 - KAUST Repository Item: Exported on 2021-02-11
Acknowledgements: The authors thank the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and the Instituto Museu Aquário Marinho do Rio de Janeiro-AquaRio (IMAM/AquaRio)—Rio de Janeiro Marine Aquarium Research Center, Rio de Janeiro.
PY - 2021/2/2
Y1 - 2021/2/2
N2 - Synthetic estrogens such as ethinylestradiol (EE2) are persistent micropollutants that are not effectively removed from wastewater by conventional treatments. These contaminants are released into waterbodies, where they disrupt endocrine systems of organisms and cause harmful effects such as feminization, infertility, reproduction problems and genital malformations. The consequences of this pollution for key marine ecosystems such as coral reefs and their associated microbiomes are underexplored. We evaluated the effects of EE2 concentrations of 100 ng L−1 and 100 µg L−1 on the coral metaorganism Mussismilia harttii. The results indicated no effects on visible bleaching or Fv/Fm ratios in the corals during a 17-day microcosm experiment. However, next-generation sequencing of 16S rDNA revealed a statistically significant effect of high EE2 concentrations on OTU richness, and shifts in specific microbial groups after treatments with or without EE2. These groups might be bioindicators of early shifts in the metaorganism composition caused by EE2 contamination. © 2021, The Author(s).
AB - Synthetic estrogens such as ethinylestradiol (EE2) are persistent micropollutants that are not effectively removed from wastewater by conventional treatments. These contaminants are released into waterbodies, where they disrupt endocrine systems of organisms and cause harmful effects such as feminization, infertility, reproduction problems and genital malformations. The consequences of this pollution for key marine ecosystems such as coral reefs and their associated microbiomes are underexplored. We evaluated the effects of EE2 concentrations of 100 ng L−1 and 100 µg L−1 on the coral metaorganism Mussismilia harttii. The results indicated no effects on visible bleaching or Fv/Fm ratios in the corals during a 17-day microcosm experiment. However, next-generation sequencing of 16S rDNA revealed a statistically significant effect of high EE2 concentrations on OTU richness, and shifts in specific microbial groups after treatments with or without EE2. These groups might be bioindicators of early shifts in the metaorganism composition caused by EE2 contamination. © 2021, The Author(s).
UR - http://hdl.handle.net/10754/667296
UR - http://www.nature.com/articles/s41598-021-82387-x
U2 - 10.1038/s41598-021-82387-x
DO - 10.1038/s41598-021-82387-x
M3 - Article
C2 - 33531587
SN - 2045-2322
VL - 11
JO - Sci. Rep.
JF - Sci. Rep.
IS - 1
ER -
