Evaluation of mucoadhesive PLGA microparticles for nasal immunization

Dilip Pawar; Amit K. Goyal; Sharad Mangal; Neeraj Mishra; Bhuvaneshwar Vaidya; Shailja Tiwari; Arvind K. Jain; Suresh P. Vyas

doi:10.1208/s12248-009-9169-1

Evaluation of mucoadhesive PLGA microparticles for nasal immunization

Dilip Pawar, Amit K. Goyal, Sharad Mangal, Neeraj Mishra, Bhuvaneshwar Vaidya, Shailja Tiwari, Arvind K. Jain, Suresh P. Vyas

Research output: Contribution to journal › Article › peer-review

79 Scopus citations

Abstract

In this study, hepatitis B surface antigen (HBsAg) loaded poly(lactic-co-glycolic acid) (PLGA) microparticles were prepared and coated with chitosan and trimethyl chitosan (TMC) to evaluate the effect of coating material for nasal vaccine delivery. The developed formulations were characterized for size, zeta potential, entrapment efficiency, and mucin adsorption ability. Plain PLGA microparticles demonstrated negative zeta potential. However, coated microparticles showed higher positive zeta potential. Results indicated that TMC microparticles demonstrated substantially higher mucin adsorption when compared to chitosan-coated microparticles and plain PLGA microparticles. The coated and uncoated microparticles showed deposition in nasal-associated lymphoid tissue under fluorescence microscopy. The coated and uncoated microparticles were then administered intranasally to mice. Immune-adjuvant effect was determined on the basis of specific antibody titer observed in serum and secretions using enzyme-linked immunosorbent assay. It was observed that coated particles showed a markedly increased anti-HBsAg titer as compared to plain PLGA microparticles, but the results were more pronounced with the TMC-coated PLGA microparticles. © 2010 American Association of Pharmaceutical Scientists.

Original language	English (US)
Pages (from-to)	130-137
Number of pages	8
Journal	AAPS Journal
Volume	12
Issue number	2
DOIs	https://doi.org/10.1208/s12248-009-9169-1
State	Published - Jun 1 2010
Externally published	Yes

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Pharmaceutical Science

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1208/s12248-009-9169-1

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title = "Evaluation of mucoadhesive PLGA microparticles for nasal immunization",

abstract = "In this study, hepatitis B surface antigen (HBsAg) loaded poly(lactic-co-glycolic acid) (PLGA) microparticles were prepared and coated with chitosan and trimethyl chitosan (TMC) to evaluate the effect of coating material for nasal vaccine delivery. The developed formulations were characterized for size, zeta potential, entrapment efficiency, and mucin adsorption ability. Plain PLGA microparticles demonstrated negative zeta potential. However, coated microparticles showed higher positive zeta potential. Results indicated that TMC microparticles demonstrated substantially higher mucin adsorption when compared to chitosan-coated microparticles and plain PLGA microparticles. The coated and uncoated microparticles showed deposition in nasal-associated lymphoid tissue under fluorescence microscopy. The coated and uncoated microparticles were then administered intranasally to mice. Immune-adjuvant effect was determined on the basis of specific antibody titer observed in serum and secretions using enzyme-linked immunosorbent assay. It was observed that coated particles showed a markedly increased anti-HBsAg titer as compared to plain PLGA microparticles, but the results were more pronounced with the TMC-coated PLGA microparticles. {\textcopyright} 2010 American Association of Pharmaceutical Scientists.",

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AU - Pawar, Dilip

AU - Goyal, Amit K.

AU - Mangal, Sharad

AU - Mishra, Neeraj

AU - Vaidya, Bhuvaneshwar

AU - Tiwari, Shailja

AU - Jain, Arvind K.

AU - Vyas, Suresh P.

N1 - Generated from Scopus record by KAUST IRTS on 2023-10-12

PY - 2010/6/1

Y1 - 2010/6/1

N2 - In this study, hepatitis B surface antigen (HBsAg) loaded poly(lactic-co-glycolic acid) (PLGA) microparticles were prepared and coated with chitosan and trimethyl chitosan (TMC) to evaluate the effect of coating material for nasal vaccine delivery. The developed formulations were characterized for size, zeta potential, entrapment efficiency, and mucin adsorption ability. Plain PLGA microparticles demonstrated negative zeta potential. However, coated microparticles showed higher positive zeta potential. Results indicated that TMC microparticles demonstrated substantially higher mucin adsorption when compared to chitosan-coated microparticles and plain PLGA microparticles. The coated and uncoated microparticles showed deposition in nasal-associated lymphoid tissue under fluorescence microscopy. The coated and uncoated microparticles were then administered intranasally to mice. Immune-adjuvant effect was determined on the basis of specific antibody titer observed in serum and secretions using enzyme-linked immunosorbent assay. It was observed that coated particles showed a markedly increased anti-HBsAg titer as compared to plain PLGA microparticles, but the results were more pronounced with the TMC-coated PLGA microparticles. © 2010 American Association of Pharmaceutical Scientists.

AB - In this study, hepatitis B surface antigen (HBsAg) loaded poly(lactic-co-glycolic acid) (PLGA) microparticles were prepared and coated with chitosan and trimethyl chitosan (TMC) to evaluate the effect of coating material for nasal vaccine delivery. The developed formulations were characterized for size, zeta potential, entrapment efficiency, and mucin adsorption ability. Plain PLGA microparticles demonstrated negative zeta potential. However, coated microparticles showed higher positive zeta potential. Results indicated that TMC microparticles demonstrated substantially higher mucin adsorption when compared to chitosan-coated microparticles and plain PLGA microparticles. The coated and uncoated microparticles showed deposition in nasal-associated lymphoid tissue under fluorescence microscopy. The coated and uncoated microparticles were then administered intranasally to mice. Immune-adjuvant effect was determined on the basis of specific antibody titer observed in serum and secretions using enzyme-linked immunosorbent assay. It was observed that coated particles showed a markedly increased anti-HBsAg titer as compared to plain PLGA microparticles, but the results were more pronounced with the TMC-coated PLGA microparticles. © 2010 American Association of Pharmaceutical Scientists.

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Evaluation of mucoadhesive PLGA microparticles for nasal immunization

Abstract

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