TY - GEN
T1 - Exploring cell structure, dynamics and homeostasis with a multimodal microscopy approach based on digital holographic microscopy
T2 - 17th Workshop on Information Optics, WIO 2018
AU - Marquet, Pierre
AU - Jourdain, Pascal
AU - Belanger, Erik
AU - Magistretti, Pierre
N1 - Funding Information:
This research has been funded by the Canada Excellence Research Chair in Neurophotonics, Université Laval, Université de Lausanne, Lausanne University Hospital and the Fondation de Préfargier.
Publisher Copyright:
© 2018 IEEE.
PY - 2019/2/15
Y1 - 2019/2/15
N2 - Among the different techniques in the growing field of quantitative phase imaging (QPI), Quantitative Phase Digital Holographic Microscopy (QP-DHM) is particularly well suited to explore, with a nanometric axial sensitivity, cell structure and dynamics, by providing quantitative phase signal (QPS). QPS depends on both the thickness and the intracellular refractive index of the observed cells and brings thus information about both cell morphology and cell contents. Thanks to the development of different experimental procedures, relevant biophysical cell parameters can be successfully calculated from QPS, including cell shape, absolute volume, intracellular protein concentration, organelle distribution, nanoscale membrane fluctuations, membrane mechanical properties and water permeability, as well as transmembrane water movements. Simultaneous dynamic imaging of transmembrane water movements and cell volume is likely to assess the cell capacity to maintain or not homeostasis and consequently to identify early biomarkers of cell viability and cytotoxicity.
AB - Among the different techniques in the growing field of quantitative phase imaging (QPI), Quantitative Phase Digital Holographic Microscopy (QP-DHM) is particularly well suited to explore, with a nanometric axial sensitivity, cell structure and dynamics, by providing quantitative phase signal (QPS). QPS depends on both the thickness and the intracellular refractive index of the observed cells and brings thus information about both cell morphology and cell contents. Thanks to the development of different experimental procedures, relevant biophysical cell parameters can be successfully calculated from QPS, including cell shape, absolute volume, intracellular protein concentration, organelle distribution, nanoscale membrane fluctuations, membrane mechanical properties and water permeability, as well as transmembrane water movements. Simultaneous dynamic imaging of transmembrane water movements and cell volume is likely to assess the cell capacity to maintain or not homeostasis and consequently to identify early biomarkers of cell viability and cytotoxicity.
KW - cell biomarkers
KW - cell imaging
KW - digital holographic microscopy
KW - glutamate
KW - quantitative phase imaging
UR - http://www.scopus.com/inward/record.url?scp=85063548060&partnerID=8YFLogxK
U2 - 10.1109/WIO.2018.8643551
DO - 10.1109/WIO.2018.8643551
M3 - Conference contribution
AN - SCOPUS:85063548060
T3 - 2018 17th Workshop on Information Optics, WIO 2018
BT - 2018 17th Workshop on Information Optics, WIO 2018
PB - Institute of Electrical and Electronics Engineers Inc.
Y2 - 16 July 2018 through 19 July 2018
ER -