Exploring cell structure, dynamics and homeostasis with a multimodal microscopy approach based on digital holographic microscopy: Towards identifying early biomarkers of cell viability and cytotoxicity

Pierre Marquet, Pascal Jourdain, Erik Belanger, Pierre Magistretti

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Among the different techniques in the growing field of quantitative phase imaging (QPI), Quantitative Phase Digital Holographic Microscopy (QP-DHM) is particularly well suited to explore, with a nanometric axial sensitivity, cell structure and dynamics, by providing quantitative phase signal (QPS). QPS depends on both the thickness and the intracellular refractive index of the observed cells and brings thus information about both cell morphology and cell contents. Thanks to the development of different experimental procedures, relevant biophysical cell parameters can be successfully calculated from QPS, including cell shape, absolute volume, intracellular protein concentration, organelle distribution, nanoscale membrane fluctuations, membrane mechanical properties and water permeability, as well as transmembrane water movements. Simultaneous dynamic imaging of transmembrane water movements and cell volume is likely to assess the cell capacity to maintain or not homeostasis and consequently to identify early biomarkers of cell viability and cytotoxicity.

Original languageEnglish (US)
Title of host publication2018 17th Workshop on Information Optics, WIO 2018
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781538660133
DOIs
StatePublished - Feb 15 2019
Event17th Workshop on Information Optics, WIO 2018 - Quebec, Canada
Duration: Jul 16 2018Jul 19 2018

Publication series

Name2018 17th Workshop on Information Optics, WIO 2018

Conference

Conference17th Workshop on Information Optics, WIO 2018
Country/TerritoryCanada
CityQuebec
Period07/16/1807/19/18

Keywords

  • cell biomarkers
  • cell imaging
  • digital holographic microscopy
  • glutamate
  • quantitative phase imaging

ASJC Scopus subject areas

  • Information Systems
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

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