TY - JOUR
T1 - Fabrication of lumen-forming colorectal cancer organoids using a newly designed lamininderived bioink
AU - Pérez-Pedroza, Rosario
AU - Al-Jalih, Fatimah
AU - Xu, Jiayi
AU - Moretti, Manola
AU - Briola, Giuseppina R.
AU - Hauser, Charlotte A.E.
N1 - Funding Information:
This work was supported by KAUST baseline funding and CBRC funding.
Publisher Copyright:
© 2022 Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution License, permitting distribution and reproduction in any medium, provided the original work is properly cited.
PY - 2023
Y1 - 2023
N2 - Three-dimensional (3D) bioprinting systems, which are the prominent tools for biofabrication, should evolve around the cutting-edge technologies of tissue engineering. This is the case with organoid technology, which requires a plethora of new materials to evolve, including extracellular matrices with specific mechanical and biochemical properties. For a bioprinting system to facilitate organoid growth, it must be able to recreate an organ-like environment within the 3D construct. In this study, a well-established, self-assembling peptide system was employed to generate a laminin-like bioink to provide signals of cell adhesion and lumen formation in cancer stem cells. One bioink formulation led to the formation of lumen with outperforming characteristics, which showed good stability of the printed construct.
AB - Three-dimensional (3D) bioprinting systems, which are the prominent tools for biofabrication, should evolve around the cutting-edge technologies of tissue engineering. This is the case with organoid technology, which requires a plethora of new materials to evolve, including extracellular matrices with specific mechanical and biochemical properties. For a bioprinting system to facilitate organoid growth, it must be able to recreate an organ-like environment within the 3D construct. In this study, a well-established, self-assembling peptide system was employed to generate a laminin-like bioink to provide signals of cell adhesion and lumen formation in cancer stem cells. One bioink formulation led to the formation of lumen with outperforming characteristics, which showed good stability of the printed construct.
KW - Biofunctional bioink
KW - Ikvav
KW - Laminin
KW - Organoid
KW - Self-assembling peptide
UR - http://www.scopus.com/inward/record.url?scp=85142280536&partnerID=8YFLogxK
U2 - 10.18063/ijb.v9i1.633
DO - 10.18063/ijb.v9i1.633
M3 - Article
C2 - 36866082
AN - SCOPUS:85142280536
SN - 2424-7723
VL - 9
SP - 160
EP - 172
JO - International Journal of Bioprinting
JF - International Journal of Bioprinting
IS - 1
ER -
