Fabrication of Potentiometric Cholesterol Biosensor by Crosslinking of Cholesterol Oxidase and Carbon Nanotubes Modified Cellulose Acetate Membrane

Anamika Pandey, Padmaker Pandey, O. P. Pandey, Nikhil Kant Shukla

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The principal objective of this work is to design a novel and improved membranous support for electrochemical sensing of cholesterol by the application of CNT. The present work is based on potentiometric sensing of cholesterol through a cellulose acetate membranous support embedded with a single walled carbon nanotubes (SWNT). Cholesterol Oxidase (ChOx) has been immobilized on the carbon nanotubes mixed cellulose acetate membrane (CA-CNT). Scanning electron microscopic images have shown that cholesterol oxidase is uniformly immobilized over the CA-CNT membrane. The electrocatalytic responses of ChOx/CA-CNT were investigated with various concentration of cholesterol ranging from 10-3 M to 10-8 M. Compared with ChOx/CA, the ChOx/CA-CNT has better electrocatalytic response to cholesterol. This sensor shows excellent performance with high sensitivity, with a linear range of 10-3 M to 10-8 M, and a detection limit of 10-8 M.
Original languageEnglish (US)
Pages (from-to)102-108
Number of pages7
JournalSensor Letters
Volume14
Issue number1
DOIs
StatePublished - Jan 1 2016
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electrical and Electronic Engineering

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