TY - JOUR
T1 - Fruity flavors from waste: A novel process to upgrade crude glycerol to ethyl valerate
AU - Ganigué, Ramon
AU - Naert, Pieter
AU - Candry, Pieter
AU - de Smedt, Jonas
AU - Stevens, Christian V.
AU - Rabaey, Korneel
N1 - KAUST Repository Item: Exported on 2020-10-01
Acknowledged KAUST grant number(s): OSR-2016-CRG5-2985
Acknowledgements: R.G. gratefully acknowledges support from Ghent University Special Research Fund (BOF) (BOF15/PDO/068). P.N. is supported by the Research Foundation Flanders (FWO-SB-151223). P.C. is supported by the Ghent University Special Research Fund (BOF) (BOF15/DOC/286). K.R. is supported by Catalisti and VLAIO-ICON “CAPRA: upgrading steel mill off gas to caproic acid and derivatives using anaerobic technology” (VLAIO HBC.2016.0413) as well as a Competitive Research Grant from King Abdullah University of Science and Technology (KAUST), Project Reference OSR-2016-CRG5-2985. Authors thank Proviron for providing the crude glycerol used in the study.
This publication acknowledges KAUST support, but has no KAUST affiliated authors.
PY - 2019/5/28
Y1 - 2019/5/28
N2 - Valeric acid and its ester derivatives are chemical compounds with a high industrial interest. Here we report a new approach to produce them from crude glycerol, by combining propionic acid fermentation with chain elongation. Propionic acid was produced by Propionibacterium acidipropionici (8.49 ± 1.40 g·L−1). In the subsequent mixed population chain elongation, valeric acid was the dominant product (5.3 ± 0.69 g·L−1) of the chain elongation process. Residual glycerol negatively impacted the selectivity of mixed culture chain elongation towards valeric acid, whereas this was unaffected when Clostridium kluyveri was used as bio-catalyst. Valeric acid could be selectively isolated and upgraded to ethyl valerate by using dodecane as extractant and medium for esterification, whereas shorter-chain carboxylic acids could be recovered by using a 10 wt% solution of trioctylphosphine oxide (TOPO) in dodecane. Overall, our work shows that the combined fermentation, electrochemistry and homogeneous catalysis enables fine chemical production from side streams.
AB - Valeric acid and its ester derivatives are chemical compounds with a high industrial interest. Here we report a new approach to produce them from crude glycerol, by combining propionic acid fermentation with chain elongation. Propionic acid was produced by Propionibacterium acidipropionici (8.49 ± 1.40 g·L−1). In the subsequent mixed population chain elongation, valeric acid was the dominant product (5.3 ± 0.69 g·L−1) of the chain elongation process. Residual glycerol negatively impacted the selectivity of mixed culture chain elongation towards valeric acid, whereas this was unaffected when Clostridium kluyveri was used as bio-catalyst. Valeric acid could be selectively isolated and upgraded to ethyl valerate by using dodecane as extractant and medium for esterification, whereas shorter-chain carboxylic acids could be recovered by using a 10 wt% solution of trioctylphosphine oxide (TOPO) in dodecane. Overall, our work shows that the combined fermentation, electrochemistry and homogeneous catalysis enables fine chemical production from side streams.
UR - http://hdl.handle.net/10754/656329
UR - https://linkinghub.elsevier.com/retrieve/pii/S0960852419308041
UR - http://www.scopus.com/inward/record.url?scp=85067674423&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2019.121574
DO - 10.1016/j.biortech.2019.121574
M3 - Article
C2 - 31247530
SN - 0960-8524
VL - 289
SP - 121574
JO - Bioresource Technology
JF - Bioresource Technology
ER -