TY - JOUR
T1 - Functional conservation of rice OsNF-YB/YC and Arabidopsis AtNF-YB/YC proteins in the regulation of flowering time
AU - Hwang, Yoon-Hyung
AU - Kim, SoonKap
AU - Lee, Keh Chien
AU - Chung, Young Soo
AU - Lee, Jeong Hwan
AU - Kim, Jeong-Kook
N1 - KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: We thank Prof. Ben F. Holt III for providing nf-yb2 nf-yb3 and nf-yc3 nf-yc4 nf-yc9 mutants and other mutants not used in this study. This work was supported by the Next-Generation BioGreen 21 Program (PJ007978), Rural Development Administration, Republic of Korea and a Korea University Grant (to J.-K. Kim), Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2015R1D1A4A0101941 to J.H. Lee and 2014R1A1A2055796 to J.-K. Kim), and by the BK 21 program (to S.-K. Kim).
PY - 2016/1/11
Y1 - 2016/1/11
N2 - Plant NUCLEAR FACTOR Y (NF-Y) transcription factors play important roles in plant development and abiotic stress. In Arabidopsis thaliana, two NF-YB (AtNF-YB2 and AtNF-YB3) and five NF-YC (AtNF-YC1, AtNF-YC2, AtNF-YC3, AtNF-YC4, and AtNF-YC9) genes regulate photoperiodic flowering by interacting with other AtNF-Y subunit proteins. Three rice NF-YB (OsNF-YB8, OsNF-YB10, and OsNF-YB11) and five rice OsNF-YC (OsNF-YC1, OsNF-YC2, OsNF-YC4, OsNF-YC6, and OsNF-YC7) genes are clustered with two AtNF-YB and five AtNF-YC genes, respectively. To investigate the functional conservation of these NF-YB and NF-YC genes in rice and Arabidopsis, we analyzed the flowering phenotypes of transgenic plants overexpressing the respective OsNF-YB and OsNF-YC genes in Arabidopsis mutants. Overexpression of OsNF-YB8/10/11 and OsNF-YC2 complemented the late flowering phenotype of Arabidopsis nf-yb2 nf-yb3 and nf-yc3 nf-yc4 nf-yc9 mutants, respectively. The rescued phenotype of 35S
AB - Plant NUCLEAR FACTOR Y (NF-Y) transcription factors play important roles in plant development and abiotic stress. In Arabidopsis thaliana, two NF-YB (AtNF-YB2 and AtNF-YB3) and five NF-YC (AtNF-YC1, AtNF-YC2, AtNF-YC3, AtNF-YC4, and AtNF-YC9) genes regulate photoperiodic flowering by interacting with other AtNF-Y subunit proteins. Three rice NF-YB (OsNF-YB8, OsNF-YB10, and OsNF-YB11) and five rice OsNF-YC (OsNF-YC1, OsNF-YC2, OsNF-YC4, OsNF-YC6, and OsNF-YC7) genes are clustered with two AtNF-YB and five AtNF-YC genes, respectively. To investigate the functional conservation of these NF-YB and NF-YC genes in rice and Arabidopsis, we analyzed the flowering phenotypes of transgenic plants overexpressing the respective OsNF-YB and OsNF-YC genes in Arabidopsis mutants. Overexpression of OsNF-YB8/10/11 and OsNF-YC2 complemented the late flowering phenotype of Arabidopsis nf-yb2 nf-yb3 and nf-yc3 nf-yc4 nf-yc9 mutants, respectively. The rescued phenotype of 35S
UR - http://hdl.handle.net/10754/596898
UR - http://link.springer.com/10.1007/s00299-015-1927-1
UR - http://www.scopus.com/inward/record.url?scp=84954140298&partnerID=8YFLogxK
U2 - 10.1007/s00299-015-1927-1
DO - 10.1007/s00299-015-1927-1
M3 - Article
C2 - 26754793
SN - 0721-7714
VL - 35
SP - 857
EP - 865
JO - Plant Cell Reports
JF - Plant Cell Reports
IS - 4
ER -