Gene Editing of Primary Rhesus Macaque B Cells

Harald Hartweger, Rajeev Gautam, Yoshiaki Nishimura, Fabian Schmidt, Kai-Hui Yao, Amelia Escolano, Mila Jankovic, Malcolm A. Martin, Michel C. Nussenzweig

Research output: Contribution to journalArticlepeer-review


B cells and their progeny are the sources of highly expressed antibodies. Their high protein expression capabilities together with their abundance, easy accessibility via peripheral blood, and amenability to simple adoptive transfers have made them an attractive target for gene editing approaches to express recombinant antibodies or other therapeutic proteins. The gene editing of mouse and human primary B cells is efficient, and mouse models for in vivo studies have shown promise, but feasibility and scalability for larger animal models have so far not been demonstrated. We, therefore, developed a protocol to edit rhesus macaque primary B cells in vitro to enable such studies. We report conditions for in vitro culture and gene-editing of primary rhesus macaque B cells from peripheral blood mononuclear cells or splenocytes using CRISPR/Cas9. To achieve the targeted integration of large (
Original languageEnglish (US)
JournalJournal of Visualized Experiments
Issue number192
StatePublished - Feb 10 2023

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)
  • Immunology and Microbiology(all)
  • Chemical Engineering(all)
  • Neuroscience(all)


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