TY - CHAP
T1 - Generation of loss-of-function mutants for wheat rust disease resistance gene cloning
AU - Mago, Rohit
AU - Till, Bradley
AU - Periyannan, Sambasivam
AU - Yu, Guotai
AU - Wulff, Brande B.H.
AU - Lagudah, Evans
N1 - Generated from Scopus record by KAUST IRTS on 2023-02-20
PY - 2017/1/1
Y1 - 2017/1/1
N2 - One of the most important tools to identify and validate rust resistance gene function is by producing lossof- function mutants. Mutants can be produced using irradiation, chemicals, and insertions. Among all the mutagens, ethyl methanesulfonate (EMS) and sodium azide are most favored because of the ease of use and generation of random point mutations in the genome. The mutants so produced facilitate the isolation, identification and cloning of rust resistance genes. In this chapter we describe a protocol for seed mutagenesis of wheat with EMS and sodium azide.
AB - One of the most important tools to identify and validate rust resistance gene function is by producing lossof- function mutants. Mutants can be produced using irradiation, chemicals, and insertions. Among all the mutagens, ethyl methanesulfonate (EMS) and sodium azide are most favored because of the ease of use and generation of random point mutations in the genome. The mutants so produced facilitate the isolation, identification and cloning of rust resistance genes. In this chapter we describe a protocol for seed mutagenesis of wheat with EMS and sodium azide.
UR - http://link.springer.com/10.1007/978-1-4939-7249-4_17
UR - http://www.scopus.com/inward/record.url?scp=85028993472&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-7249-4_17
DO - 10.1007/978-1-4939-7249-4_17
M3 - Chapter
SP - 199
EP - 205
BT - Methods in Molecular Biology
PB - Humana Press [email protected]
ER -