TY - JOUR
T1 - Generation of mouse-induced pluripotent stem cells by transient expression of a single nonviral polycistronic vector
AU - Gonzalez, Federico
AU - Monasterio, Montserrat Barragan
AU - Tiscornia, Gustavo
AU - Pulido, Nuria Montserrat
AU - Vassena, Rita
AU - Morera, Laura Batlle
AU - Piza, Ignasi Rodriguez
AU - Belmonte, Juan Carlos Izpisua
PY - 2009/6/2
Y1 - 2009/6/2
N2 - Induced pluripotent stem (iPS) cells have generated keen interest due to their potential use in regenerative medicine. They have been obtained from various cell types of both mice and humans by exogenous delivery of different combinations of Oct4, Sox2, Klf4, c-Myc, Nanog, and Lin28. The delivery of these transcription factors has mostly entailed the use of integrating viral vectors (retroviruses or lentiviruses), carrying the risk of both insertional mutagenesis and oncogenesis due to misexpression of these exogenous factors. Therefore, obtaining iPS cells that do not carry integrated transgene sequences is an important prerequisite for their eventual therapeutic use. Here we report the generation of iPS cell lines from mouse embryonic fibroblasts with no evidence of integration of the reprogramming vector in their genome, achieved by nucleofection of a polycistronic construct coexpressing Oct4, Sox2, Klf4, and c-Myc.
AB - Induced pluripotent stem (iPS) cells have generated keen interest due to their potential use in regenerative medicine. They have been obtained from various cell types of both mice and humans by exogenous delivery of different combinations of Oct4, Sox2, Klf4, c-Myc, Nanog, and Lin28. The delivery of these transcription factors has mostly entailed the use of integrating viral vectors (retroviruses or lentiviruses), carrying the risk of both insertional mutagenesis and oncogenesis due to misexpression of these exogenous factors. Therefore, obtaining iPS cells that do not carry integrated transgene sequences is an important prerequisite for their eventual therapeutic use. Here we report the generation of iPS cell lines from mouse embryonic fibroblasts with no evidence of integration of the reprogramming vector in their genome, achieved by nucleofection of a polycistronic construct coexpressing Oct4, Sox2, Klf4, and c-Myc.
KW - Nonintegrative technique
KW - Pluripotency
KW - Reprogramming
KW - iPS cells
UR - http://www.scopus.com/inward/record.url?scp=67049155513&partnerID=8YFLogxK
U2 - 10.1073/pnas.0901471106
DO - 10.1073/pnas.0901471106
M3 - Article
C2 - 19458047
AN - SCOPUS:67049155513
SN - 0027-8424
VL - 106
SP - 8918
EP - 8922
JO - PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
JF - PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
IS - 22
ER -