Generation of myoglobin (MB)-knockout human embryonic stem cell (hESC) line (KAIMRCe002-A-1S) using CRISPR/Cas9 technology.

Maryam Alowaysi, Mohammad Al-Shehri, Moayad Baadhaim, Hajar AlZahrani, Doaa Aboalola, Mustafa Daghestani, Heba Hashem, Rawan Aljahdali, Rayan Salem, Adel Alharbi, Mohammed Muharraq, Khaled Alghamdi, Fawaz Alsobiy, Asima Zia, Robert Lehmann, Jesper Tegner, Khaled Alsayegh

Research output: Contribution to journalArticlepeer-review


Myoglobin (MB) is a cytoplasmic hemoprotein that is predominantly expressed in the heart and oxidative myofibers of skeletal muscle. It has been demonstrated that MB binds to oxygen and promotes its diffusion for energy production in the mitochondria. Recently, MB was found to be expressed in different forms of malignant tumors and cancer cell lines. Further studies using gene disruption technology will enhance the understanding of MB’s role in human cardiovascular biology and cancers. Here, we describe the generation of a homozygous MB knockout in human embryonic stem cells (hESC-MB−/−) via CRISPR/Cas9 to study MB function in human biology and diseases.
Original languageEnglish (US)
Pages (from-to)103158
JournalStem Cell Research
StatePublished - Jul 3 2023

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • General Medicine


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