TY - JOUR
T1 - Genetic analysis of RNA-mediated gene silencing in Arabidopsis thaliana
AU - Finke, Andreas
AU - Mette, Michael Florian
AU - Kuhlmann, Markus
N1 - Funding Information:
Acknowledgments We thank Christa Fricke, Inge Glaser and Beate Kamm for excellent technical assistance. This work received support from IPK Gatersleben (to A.F.) and the German Research Foundation (DFG) collaborative research centre (SFB) 648 ‘‘Molecular Mechanisms of Information Processing in Plants’’ (to M.K.).
PY - 2012/3
Y1 - 2012/3
N2 - In plants, a particular class of small non-coding RNAs, short interfering RNAs, can serve as a signal to induce cytosine methylation at homologous genomic DNA regions in the nucleus. If the targeted DNA regions have promoter function, this RNA-directed DNA methylation (RdDM) can result in transcriptional gene silencing (TGS). RNA-directed transcriptional gene silencing of transgenes provides a versatile system for the study of epigenetic gene regulation in plants. In our experimental setup in Arabidopsis thaliana, transcription of a promoter-inverted repeat provides a RNA signal that triggers de novo cytosine methylation and TGS of a homologous nopaline synthase promoter (proNOS) in trans. Utilising this two component transgene system in a forward-genetic screen for "suppressor of silencing" mutations, we were able to identify new candidates for factors involved in RdDM of transgenic as well as endogenous target regions.
AB - In plants, a particular class of small non-coding RNAs, short interfering RNAs, can serve as a signal to induce cytosine methylation at homologous genomic DNA regions in the nucleus. If the targeted DNA regions have promoter function, this RNA-directed DNA methylation (RdDM) can result in transcriptional gene silencing (TGS). RNA-directed transcriptional gene silencing of transgenes provides a versatile system for the study of epigenetic gene regulation in plants. In our experimental setup in Arabidopsis thaliana, transcription of a promoter-inverted repeat provides a RNA signal that triggers de novo cytosine methylation and TGS of a homologous nopaline synthase promoter (proNOS) in trans. Utilising this two component transgene system in a forward-genetic screen for "suppressor of silencing" mutations, we were able to identify new candidates for factors involved in RdDM of transgenic as well as endogenous target regions.
KW - Arabidopsis thaliana
KW - Mutagenesis
KW - NOS promoter
KW - RNA-dependent DNA methylation
KW - Transcriptional gene silencing
UR - http://www.scopus.com/inward/record.url?scp=84857649435&partnerID=8YFLogxK
U2 - 10.1007/s00003-011-0754-8
DO - 10.1007/s00003-011-0754-8
M3 - Article
AN - SCOPUS:84857649435
SN - 1661-5751
VL - 7
SP - 27
EP - 33
JO - Journal fur Verbraucherschutz und Lebensmittelsicherheit
JF - Journal fur Verbraucherschutz und Lebensmittelsicherheit
IS - 1
ER -