Graphene-based high-efficiency surface-enhanced Raman scattering-active platform for sensitive and multiplex DNA detection

Shijiang He; Keng Ku Liu; Shao Su; Juan Yan; Xiuhai Mao; Dongfang Wang; Yao He; Lain Jong Li; Shiping Song; Chunhai Fan

doi:10.1021/ac300577d

Graphene-based high-efficiency surface-enhanced Raman scattering-active platform for sensitive and multiplex DNA detection

Shijiang He, Keng Ku Liu, Shao Su, Juan Yan, Xiuhai Mao, Dongfang Wang, Yao He, Lain Jong Li, Shiping Song^*, Chunhai Fan

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

186 Scopus citations

Abstract

We have developed a surface-enhanced Raman scattering (SERS)-active substrate based on gold nanoparticle-decorated chemical vapor deposition (CVD)-growth graphene and used it for multiplexing detection of DNA. Due to the combination of gold nanoparticles and graphene, the Raman signals of dye were dramatically enhanced by this novel substrate. With the gold nanoparticles, DNA capture probes could be easily assembled on the surface of graphene films which have a drawback to directly immobilize DNA. This platform exhibits extraordinarily high sensitivity and excellent specificity for DNA detection. A detection limit as low as 10 pM is obtained. Importantly, two different DNA targets could be detected simultaneously on the same substrate just using one light source.

Original language	English (US)
Pages (from-to)	4622-4627
Number of pages	6
Journal	ANALYTICAL CHEMISTRY
Volume	84
Issue number	10
DOIs	https://doi.org/10.1021/ac300577d
State	Published - May 15 2012
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry

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title = "Graphene-based high-efficiency surface-enhanced Raman scattering-active platform for sensitive and multiplex DNA detection",

abstract = "We have developed a surface-enhanced Raman scattering (SERS)-active substrate based on gold nanoparticle-decorated chemical vapor deposition (CVD)-growth graphene and used it for multiplexing detection of DNA. Due to the combination of gold nanoparticles and graphene, the Raman signals of dye were dramatically enhanced by this novel substrate. With the gold nanoparticles, DNA capture probes could be easily assembled on the surface of graphene films which have a drawback to directly immobilize DNA. This platform exhibits extraordinarily high sensitivity and excellent specificity for DNA detection. A detection limit as low as 10 pM is obtained. Importantly, two different DNA targets could be detected simultaneously on the same substrate just using one light source.",

author = "Shijiang He and Liu, {Keng Ku} and Shao Su and Juan Yan and Xiuhai Mao and Dongfang Wang and Yao He and Li, {Lain Jong} and Shiping Song and Chunhai Fan",

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AU - He, Shijiang

AU - Liu, Keng Ku

AU - Su, Shao

AU - Yan, Juan

AU - Mao, Xiuhai

AU - Wang, Dongfang

AU - He, Yao

AU - Li, Lain Jong

AU - Song, Shiping

AU - Fan, Chunhai

PY - 2012/5/15

Y1 - 2012/5/15

N2 - We have developed a surface-enhanced Raman scattering (SERS)-active substrate based on gold nanoparticle-decorated chemical vapor deposition (CVD)-growth graphene and used it for multiplexing detection of DNA. Due to the combination of gold nanoparticles and graphene, the Raman signals of dye were dramatically enhanced by this novel substrate. With the gold nanoparticles, DNA capture probes could be easily assembled on the surface of graphene films which have a drawback to directly immobilize DNA. This platform exhibits extraordinarily high sensitivity and excellent specificity for DNA detection. A detection limit as low as 10 pM is obtained. Importantly, two different DNA targets could be detected simultaneously on the same substrate just using one light source.

AB - We have developed a surface-enhanced Raman scattering (SERS)-active substrate based on gold nanoparticle-decorated chemical vapor deposition (CVD)-growth graphene and used it for multiplexing detection of DNA. Due to the combination of gold nanoparticles and graphene, the Raman signals of dye were dramatically enhanced by this novel substrate. With the gold nanoparticles, DNA capture probes could be easily assembled on the surface of graphene films which have a drawback to directly immobilize DNA. This platform exhibits extraordinarily high sensitivity and excellent specificity for DNA detection. A detection limit as low as 10 pM is obtained. Importantly, two different DNA targets could be detected simultaneously on the same substrate just using one light source.

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Graphene-based high-efficiency surface-enhanced Raman scattering-active platform for sensitive and multiplex DNA detection

Abstract

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