Green synthesized gold nanoparticles decorated graphene oxide for sensitive determination of chloramphenicol in milk, powdered milk, honey and eye drops

R. Karthik, Mani Govindasamy, Shen Ming Chen, Veerappan Mani, Bih Show Lou, Rajkumar Devasenathipathy, Yu Shen Hou, A. Elangovan

Research output: Contribution to journalArticlepeer-review

139 Scopus citations

Abstract

A simple and rapid green synthesis using Bischofia javanica Blume leaves as reducing agent was developed for the preparation of gold nanoparticles (AuNPs). AuNPs decorated graphene oxide (AuNPs/GO) was prepared and employed for the sensitive amperometric determination of chloramphenicol. The green biosynthesis requires less than 40 s to reduce gold salts to AuNPs. The formations of AuNPs and AuNPs/GO were evaluated by scanning electron and atomic force microscopies, UV-Visible and energy dispersive X-ray spectroscopies, X-ray diffraction studies, and electrochemical methods. AuNPs/GO composite film modified electrode was fabricated and shown excellent electrocatalytic ability towards chloramphenicol. Under optimal conditions, the amperometric sensing platform has delivered wide linear range of 1.5-2.95 μM, low detection limit of 0.25 μM and high sensitivity of 3.81 μA μM-1 cm-2. The developed sensor exhibited good repeatability and reproducibility, anti-interference ability and long-term storage stability. Practical feasibility of the sensor has been demonstrated in food samples (milk, powdered milk and honey) and pharmaceutical sample (eye drops). The green synthesized AuNPs/GO composite has great potential for analysis of food samples in food safety measures.
Original languageEnglish (US)
Pages (from-to)46-56
Number of pages11
JournalJournal of Colloid and Interface Science
Volume475
DOIs
StatePublished - Aug 1 2016
Externally publishedYes

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