High resolution patterning of hydrogel sensing motifs within fibrous substrates for highly sensitive and multiplexed detection of nucleic acid biomarkers

Dana Al Sulaiman; Sarah J. Shapiro; Jose Gomez-Marquez; Patrick S. Doyle

High resolution patterning of hydrogel sensing motifs within fibrous substrates for highly sensitive and multiplexed detection of nucleic acid biomarkers

Dana Al Sulaiman, Sarah J. Shapiro, Jose Gomez-Marquez, Patrick S. Doyle

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

There has been an increasing demand to develop nucleic acid bioassays which offer high analytical performance in addition to amenability with point-of-care testing. By leveraging projection lithography, we develop a biosensing platform based on direct, high-resolution polymerization of microscale hydrogel motifs within fibrous substrates. Our miniaturized platform enables multiplex detection (through spatial encoding) in a user-friendly format. As a proof-of-concept, we apply the assay to the detection of microRNA biomarkers, demonstrating a large dynamic range (>3 order of magnitude), low limit-of-detection (50 fM; 2.5amol), and compatibility with clinical applications.

Original language	English (US)
Title of host publication	MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Pages	548-549
Number of pages	2
ISBN (Print)	9781733419017
State	Published - Jan 1 2020
Externally published	Yes

Cite this

Al Sulaiman, D., Shapiro, S. J., Gomez-Marquez, J., & Doyle, P. S. (2020). High resolution patterning of hydrogel sensing motifs within fibrous substrates for highly sensitive and multiplexed detection of nucleic acid biomarkers. In MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 548-549). Chemical and Biological Microsystems Society.

Al Sulaiman, Dana ; Shapiro, Sarah J. ; Gomez-Marquez, Jose et al. / High resolution patterning of hydrogel sensing motifs within fibrous substrates for highly sensitive and multiplexed detection of nucleic acid biomarkers. MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2020. pp. 548-549

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Al Sulaiman, D, Shapiro, SJ, Gomez-Marquez, J & Doyle, PS 2020, High resolution patterning of hydrogel sensing motifs within fibrous substrates for highly sensitive and multiplexed detection of nucleic acid biomarkers. in MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, pp. 548-549.

High resolution patterning of hydrogel sensing motifs within fibrous substrates for highly sensitive and multiplexed detection of nucleic acid biomarkers. / Al Sulaiman, Dana; Shapiro, Sarah J.; Gomez-Marquez, Jose et al.
MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2020. p. 548-549.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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AB - There has been an increasing demand to develop nucleic acid bioassays which offer high analytical performance in addition to amenability with point-of-care testing. By leveraging projection lithography, we develop a biosensing platform based on direct, high-resolution polymerization of microscale hydrogel motifs within fibrous substrates. Our miniaturized platform enables multiplex detection (through spatial encoding) in a user-friendly format. As a proof-of-concept, we apply the assay to the detection of microRNA biomarkers, demonstrating a large dynamic range (>3 order of magnitude), low limit-of-detection (50 fM; 2.5amol), and compatibility with clinical applications.

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Al Sulaiman D, Shapiro SJ, Gomez-Marquez J, Doyle PS. High resolution patterning of hydrogel sensing motifs within fibrous substrates for highly sensitive and multiplexed detection of nucleic acid biomarkers. In MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2020. p. 548-549

High resolution patterning of hydrogel sensing motifs within fibrous substrates for highly sensitive and multiplexed detection of nucleic acid biomarkers

Abstract

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