TY - JOUR
T1 - High Sensitivity, High Selectivity SERS Detection of MnSOD Using Optical Nanoantennas Functionalized with Aptamers
AU - Cottat, Maximilien
AU - D'andrea, Cristiano
AU - Yasukuni, Ryohei
AU - Malashikhina, Natalia
AU - Grinyte, Ruta
AU - Lidgi-Guigui, Nathalie
AU - Fazio, Barbara
AU - Sutton, Angela
AU - Oudar, Olivier
AU - Charnaux, Nathalie
AU - Pavlov, Valery
AU - Toma, Andrea
AU - Di Fabrizio, Enzo
AU - Gucciardi, Pietro G.
AU - Lamy De La Chapelle, Marc
N1 - Publisher Copyright:
© 2015 American Chemical Society.
PY - 2015/7/9
Y1 - 2015/7/9
N2 - In this paper, we present the development of a highly sensitive, specific and reproducible nanobiosensor to detect one specific liver cancer biomarker, the manganese super oxide dismutase (MnSOD). The high sensitivity and reproducibility was reached by using SERS on gold nanostructures (nanocylinders and coupled nanorods) produced by electron-beam lithography (EBL). The specificity of the detection was provided by the use of a specific aptamer with high affinity to the targeted protein as a recognition element. With such a sensor, we have been able to observe the SERS signal of the MnSOD at concentrations down to the nM level and to show with negative control that this detection is specific due to the use of the aptamer. This latter issue has allowed us to detect the MnSOD in different body fluids (serum and saliva) at concentrations in the nM range. We have then demonstrated the effectiveness of our SERS nanobiosensor using aptamer as a bioreceptor for the detection of disease biomarker at low concentration and in complex fluids.
AB - In this paper, we present the development of a highly sensitive, specific and reproducible nanobiosensor to detect one specific liver cancer biomarker, the manganese super oxide dismutase (MnSOD). The high sensitivity and reproducibility was reached by using SERS on gold nanostructures (nanocylinders and coupled nanorods) produced by electron-beam lithography (EBL). The specificity of the detection was provided by the use of a specific aptamer with high affinity to the targeted protein as a recognition element. With such a sensor, we have been able to observe the SERS signal of the MnSOD at concentrations down to the nM level and to show with negative control that this detection is specific due to the use of the aptamer. This latter issue has allowed us to detect the MnSOD in different body fluids (serum and saliva) at concentrations in the nM range. We have then demonstrated the effectiveness of our SERS nanobiosensor using aptamer as a bioreceptor for the detection of disease biomarker at low concentration and in complex fluids.
UR - http://www.scopus.com/inward/record.url?scp=84936855155&partnerID=8YFLogxK
U2 - 10.1021/acs.jpcc.5b03681
DO - 10.1021/acs.jpcc.5b03681
M3 - Article
AN - SCOPUS:84936855155
SN - 1932-7447
VL - 119
SP - 15532
EP - 15540
JO - JOURNAL OF PHYSICAL CHEMISTRY C
JF - JOURNAL OF PHYSICAL CHEMISTRY C
IS - 27
ER -
