Highly sensitive fluorescence detection system for microfluidic lab-on-a-chip

Gihan Ryu; Jingsong Huang; Oliver Hofmann; Claire A. Walshe; Jasmine Y.Y. Sze; Gareth D. McClean; Alan Mosley; Simon J. Rattle; John C. Demello; Andrew J. Demello; Donal D.C. Bradley

doi:10.1039/c0lc00586j

Highly sensitive fluorescence detection system for microfluidic lab-on-a-chip

Gihan Ryu, Jingsong Huang, Oliver Hofmann, Claire A. Walshe, Jasmine Y.Y. Sze, Gareth D. McClean, Alan Mosley, Simon J. Rattle, John C. Demello, Andrew J. Demello, Donal D.C. Bradley

Research output: Contribution to journal › Article › peer-review

86 Scopus citations

Abstract

We demonstrate a compact, low cost and practical fluorescence detection system for lab-on-a-chip applications. The system comprises a commercially available InGaN light emitting diode (501 nm) as light source, an organic or silicon photodiode detector, absorptive dye coated colour filters and linear and reflective polarisers. An injection moulded polystyrene microfluidic chip is used as the platform for fluorescence immunoassays for cardiac markers myoglobin and CK-MB. The optical limit of detection (LOD) is measured using a TransFluoSphere® suspension at 5.6 × 104 beads l -1 which can be equated to ∼3 nM fluorescein equivalent concentration. The LOD for the human plasma immunoassays is measured as 1.5 ng ml-1 for both myoglobin and CK-MB. © 2011 The Royal Society of Chemistry.

Original language	English (US)
Journal	Lab on a Chip
Volume	11
Issue number	9
DOIs	https://doi.org/10.1039/c0lc00586j
State	Published - May 7 2011
Externally published	Yes

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title = "Highly sensitive fluorescence detection system for microfluidic lab-on-a-chip",

abstract = "We demonstrate a compact, low cost and practical fluorescence detection system for lab-on-a-chip applications. The system comprises a commercially available InGaN light emitting diode (501 nm) as light source, an organic or silicon photodiode detector, absorptive dye coated colour filters and linear and reflective polarisers. An injection moulded polystyrene microfluidic chip is used as the platform for fluorescence immunoassays for cardiac markers myoglobin and CK-MB. The optical limit of detection (LOD) is measured using a TransFluoSphere{\textregistered} suspension at 5.6 × 104 beads l -1 which can be equated to ∼3 nM fluorescein equivalent concentration. The LOD for the human plasma immunoassays is measured as 1.5 ng ml-1 for both myoglobin and CK-MB. {\textcopyright} 2011 The Royal Society of Chemistry.",

author = "Gihan Ryu and Jingsong Huang and Oliver Hofmann and Walshe, {Claire A.} and Sze, {Jasmine Y.Y.} and McClean, {Gareth D.} and Alan Mosley and Rattle, {Simon J.} and Demello, {John C.} and Demello, {Andrew J.} and Bradley, {Donal D.C.}",

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AU - Ryu, Gihan

AU - Huang, Jingsong

AU - Hofmann, Oliver

AU - Walshe, Claire A.

AU - Sze, Jasmine Y.Y.

AU - McClean, Gareth D.

AU - Mosley, Alan

AU - Rattle, Simon J.

AU - Demello, John C.

AU - Demello, Andrew J.

AU - Bradley, Donal D.C.

N1 - Generated from Scopus record by KAUST IRTS on 2019-11-27

PY - 2011/5/7

Y1 - 2011/5/7

N2 - We demonstrate a compact, low cost and practical fluorescence detection system for lab-on-a-chip applications. The system comprises a commercially available InGaN light emitting diode (501 nm) as light source, an organic or silicon photodiode detector, absorptive dye coated colour filters and linear and reflective polarisers. An injection moulded polystyrene microfluidic chip is used as the platform for fluorescence immunoassays for cardiac markers myoglobin and CK-MB. The optical limit of detection (LOD) is measured using a TransFluoSphere® suspension at 5.6 × 104 beads l -1 which can be equated to ∼3 nM fluorescein equivalent concentration. The LOD for the human plasma immunoassays is measured as 1.5 ng ml-1 for both myoglobin and CK-MB. © 2011 The Royal Society of Chemistry.

AB - We demonstrate a compact, low cost and practical fluorescence detection system for lab-on-a-chip applications. The system comprises a commercially available InGaN light emitting diode (501 nm) as light source, an organic or silicon photodiode detector, absorptive dye coated colour filters and linear and reflective polarisers. An injection moulded polystyrene microfluidic chip is used as the platform for fluorescence immunoassays for cardiac markers myoglobin and CK-MB. The optical limit of detection (LOD) is measured using a TransFluoSphere® suspension at 5.6 × 104 beads l -1 which can be equated to ∼3 nM fluorescein equivalent concentration. The LOD for the human plasma immunoassays is measured as 1.5 ng ml-1 for both myoglobin and CK-MB. © 2011 The Royal Society of Chemistry.

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VL - 11

JO - Lab on a Chip

JF - Lab on a Chip

IS - 9

ER -

Highly sensitive fluorescence detection system for microfluidic lab-on-a-chip

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